文章建立了一种利用超高效液相色谱测定番茄中番茄红素含量的方法。2。00 g 样品加入 10 mL 丙酮，涡旋混匀提取 10 min，4℃10 000 r·min-1 离心 5 min，取上层清液过 0。22 μm微孔滤膜，上机检测。结果发现，番茄红素在 1。00～100。00 μg·mL-1 浓度范围呈良好的线性关系，加标回收率为 91。2%～106。7%，相对标准偏差(RSD)为 2。8%～5。1%。该方法可以节省大量的时间和试剂，使前处理更简单，更经济，更环保。
Determination of lycopene content in tomato by ultra-high performance liquid chromatography
In this paper,a method for the determination of lycopene content in tomato by ultra-high performance liquid chromatography(UPLC)was established.10 mL of acetone was added to the 2.00 g sample,mixed with a whirl instrument for 10 min,centrifuged at 4℃at 10 000 r·min-1 for 5 min,and the supernatant was passed through a 0.22 μm microporous filter membrane for detection.The results showed that lycopene showed a good linear relationship in the concentration range of 1.00-100.00 μg·mL-1,and the recovery rate was 91.2%-106.7%,and the relative standard deviation(RSD)was 2.8%-5.1%.This method can save a lot of time and reagents,and make the pretreatment simpler,more economical,and more environmentally friendly.
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