Purification technology of total flavonoids from Tetrastigma hemsleyanum
In order to optimize the process conditions for the separation and purification of total flavonoids from polyamide,the content of total flavonoids in Tetrastigma hemsleyanum was determined by ultraviolet spectrophotometry,and the flavonoids were adsorbed by polyamide resin,and the static and dynamic adsorption and desorption properties were studied from three aspects:static and dynamic adsorption and desorption performance after different treatments.The results showed that the optimal conditions for the adsorption of total flavonoids in the leaves of Tetrastigma hemsleyanum were 15 mg·mL-1 and 1.75 g of polyamide in the loading solution,and the pH value was controlled at about 3.0 for static adsorption,and the highest adsorption rate was 76.7% .The polyamide resin was then desorbed with 60% ethanol with a desorption rate of up to 75.9% .
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