HPLC fingerprint and content analysis of adenosine and uridine in Ganoderma lucidum
The fingerprint spectrum were used to detect and determine the content of adenosine and uridine in water-soluble components of Ganoderma lucidum,in order to find out the variation of the content of different spore powders and fruiting bodies.The results showed that there were significant differences in fingerprints between Ganoderma lucidum powder and spore powder from different sources;the content of uridine in the pileus of Ganoderma lucidum was 0.072-0.849 mg·g-1,the content of uridine in the stipe was 0.056-0.302 mg·g-1,and the content of uridine in the broken spore powder was 0.032-0.089 mg·g-1;the content of adenosine in the pileus of Ganoderma lucidum was 0.019-0.118 mg·g-1,the content of adenosine in the stipe was 0.005-0.024 mg·g-1,and the content of adenosine in the broken spore powder is 0.024-0.107 mg·g-1;There was no significant difference in the content of the main components of the unbroken spore powder harvested from the same variety but with different tides.There was no significant difference between the broken spore powder and the unbroken spore powder of the same variety.The difference of adenosine and uridine content in different batches of the same variety was obvious.The identification of broken spore powder can be achieved by using the fingerprint spectrum of high-performance liquid chromatography(HPLC)with only one peak in the area of 8.5-20.0 minutes for broken spore powder.The content of uridine and adenosine is used to identify whether the spore powder contains Ganoderma lucidum powder.
万方数据
维普
