In order to obtain antagonistic bacteria for the biocontrol of tea anthracnose,63 strains of bacteria were isolated and purified from the rhizosphere soil of tea plants by dilution plate method.Among these isolates,strain HZ-01 showed the highest inhibition rate(71.5% ).Combined with morphological characteristics,physiological and biochemical characteristics and 16S rDNA sequence comparison analysis,strain HZ-01 was identified as Bacillus velezensis.Extracellular hydrolase activity analysis showed that strain HZ-01 could produce β-1,3-glucanase,protease and cellulase.The fermentation products had an obvious inhibitory effect on the mycelial growth and spore germination of Colletotrichum camelliae.The above results indicated that strain HZ-01 could be used as a candidate strain for biological control of tea anthracnose.
关键词
茶树炭疽菌/芽孢杆菌/分离鉴定/生物防治
Key words
Colletotrichum camelliae/Bacillus/isolation and identification/biological control
维普
万方数据