Screening,identification and antagonistic mechanism for biological control of tea anthracnose
In order to obtain antagonistic bacteria for the biocontrol of tea anthracnose,63 strains of bacteria were isolated and purified from the rhizosphere soil of tea plants by dilution plate method.Among these isolates,strain HZ-01 showed the highest inhibition rate(71.5% ).Combined with morphological characteristics,physiological and biochemical characteristics and 16S rDNA sequence comparison analysis,strain HZ-01 was identified as Bacillus velezensis.Extracellular hydrolase activity analysis showed that strain HZ-01 could produce β-1,3-glucanase,protease and cellulase.The fermentation products had an obvious inhibitory effect on the mycelial growth and spore germination of Colletotrichum camelliae.The above results indicated that strain HZ-01 could be used as a candidate strain for biological control of tea anthracnose.
Colletotrichum camelliaeBacillusisolation and identificationbiological control