Simultaneous detection of chlorothalonil and cyproconazole by high-performance liquid chromatography
Chlorothalonil and cyproconazole are both efficient fungicides,but there is currently no analytical method for simultaneously detecting these two components.This study aims to establish an analytical method that can simultaneously detect chlorothalonil and cyproconazole.A high-performance liquid chromatography(HPLC)was used to configure an ODS-3 C18 column and a UV detector.Methanol and water were used as mobile phases,and chlorothalonil and cyproconazole were separated and quantitatively analyzed in a 40.5% chlorothalonil cyproconazole suspension at a wavelength of 224 nm.The results showed that the linear correlation coefficients of this method for chlorothalonil and cyproconazole were 0.999 7 and 0.999 9,respectively,with standard deviations of 0.132 0 and 0.013 7,coefficient of variation of 0.35% and 0.45%,and average recovery rates of 99.34% and 99.62%,respectively.This method has good precision and accuracy,and can simultaneously detect chlorothalonil and cyproconazole in 40.5% suspension of chlorothalonil and cyproconazole,and is suitable for the detection of related products.