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高效液相色谱法同时检测百菌清和环丙唑醇

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百菌清和环丙唑醇都是高效的杀菌剂,但目前还没有同时检测这两种成分的分析方法。该研究为建立可以同时检测百菌清和环丙唑醇的分析方法,采用高效液相色谱仪配置 ODS-3 C18 色谱柱和紫外检测器,以甲醇和水为流动相,在 224 nm波长下,对 40。5%百菌清·环丙唑醇悬浮剂中百菌清和环丙唑醇进行分离和定量分析。结果表明,该方法对百菌清、环丙唑醇的线性相关系数分别为 0。999 7 和 0。999 9,标准偏差分别为0。132 0 和 0。013 7,变异系数分别为 0。35%和 0。45%,平均回收率分别为 99。34%和 99。62%。该方法具有良好的精密度和准确度,可以同时检测 40。5%百菌清·环丙唑醇悬浮剂中的百菌清和环丙唑醇,且适用于相关产品的检测。
Simultaneous detection of chlorothalonil and cyproconazole by high-performance liquid chromatography
Chlorothalonil and cyproconazole are both efficient fungicides,but there is currently no analytical method for simultaneously detecting these two components.This study aims to establish an analytical method that can simultaneously detect chlorothalonil and cyproconazole.A high-performance liquid chromatography(HPLC)was used to configure an ODS-3 C18 column and a UV detector.Methanol and water were used as mobile phases,and chlorothalonil and cyproconazole were separated and quantitatively analyzed in a 40.5% chlorothalonil cyproconazole suspension at a wavelength of 224 nm.The results showed that the linear correlation coefficients of this method for chlorothalonil and cyproconazole were 0.999 7 and 0.999 9,respectively,with standard deviations of 0.132 0 and 0.013 7,coefficient of variation of 0.35% and 0.45%,and average recovery rates of 99.34% and 99.62%,respectively.This method has good precision and accuracy,and can simultaneously detect chlorothalonil and cyproconazole in 40.5% suspension of chlorothalonil and cyproconazole,and is suitable for the detection of related products.

chlorothalonilcyproconazolesuspensionhigh-performance liquid chromatographyanalysis

龚国华

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浙江省龙游东方阿纳萨克作物科技有限公司,浙江 衢州 324000

百菌清 环丙唑醇 悬浮剂 高效液相色谱 分析

2024

浙江农业科学
浙江省农业科学院,浙江大学

浙江农业科学

影响因子:0.523
ISSN:0528-9017
年,卷(期):2024.65(8)