Isolation,virulence gene identification,and antimicrobial resistance analysis of hybrid enterotoxigenic and Shiga toxin-producing Escherichia coli(ETEC/STEC)strain from pigs
Post weaning diarrhea(PWD)in piglets is usually caused by infection with enterotoxigenic Escherichia coli(ETEC),which typically carries one or more of the enterotoxins LT,STa,and STb.Pigt edema disease(ED),also known as bowel edema or gut edema,is an acute and lethal infectious disease caused by infection with Shiga toxin-producing Escherichia coli(STEC),with a high mortality rate.This bacterium usually carries toxins such as Stx1 and Stx2.In this study,we diagnosed a case of PWD complicated by ED with high mortality in piglets,and isolated and identified a hybrid strain carrying both enterotoxigenic Escherichia coli(ETEC)and Shiga toxin-producing Escherichia coli(STEC)virulence genes.It shows that the hybrid strain carried the F18 adhesin gene,enterotoxin genes(STa,STb,LT),and edema disease causing toxin gene(Stx2e).The results of antimicrobial sensitivity test showed that the E.coli isolate was sensitive to five drugs,including cefotaxime and amikacin,moderately sensitive to two drugs,including spectinomycin and ciprofloxacin,and resistant to 14 drugs,including enrofloxacin and streptomycin.The isolate is a hybrid Escherichia coli(ETEC/STEC),and shows strong resistance to multiple antibiotics.
post weaning diarrheapig edema diseaseisolation and identificationvirulence gene
万方数据
维普
