Efficient regeneration system of Peucedanum praeruptorum Dunn based on adventitious bud induction
To establish an efficient regeneration system of Peucedanum praeruptorum Dunn,the effects of different concentrations of hormones on the induction and rooting of adventitious buds were studied with the stem tip tissues of wild Peucedanum praeruptorum Dunn as explants.The results showed that the best medium for adventitious bud induction was MS+2.5 mg·L-1 6-BA,the induction rate reached 100%,and the multiplication rate of adventitious buds was 7.3 times.The best rooting medium was 1/2MS+0.2 mg·L-1 NAA.The best transplanting method was to transplant the seedlings to the seedbed after 30 days-hydroponic training of the plants which are higher than 3 cm,with 3 to 5 roots over 2 cm.This system provides a new way for tissue culture and regeneration of Peucedanum praeruptorum Dunn and shortens the period of seedling breeding.
Peucedanum praeruptorum Dunntissue cultureadventitious budsregeneration system
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