桃蚜(Myzus persicae)是桃树上最重要的害虫之一，为明确其寄生性天敌的物种多样性，该研究对江苏沿海地区桃园内桃蚜僵蚜进行采集，待僵蚜中寄生蜂成虫羽化后采用形态学和 DNA 条形码的方法相结合进行鉴定。目前调查共发现该地区桃蚜寄生蜂有 2 科 5 种，分别为蚜小蜂科(Aphelinidae)的短矩蚜小蜂(Aphelinus abdominalis)和褐带蚜小蜂(Aphelinus maculatus);茧蜂科(Braconidae)的细长径蚜茧蜂(Lipolexis gracilis)、棉蚜茧蜂(Binodoxys communis)和烟蚜茧蜂(Aphidius gifuensis)。该研究提取了 5 种寄生蜂的 COI 基因，通过构建进化树的方法，发现分子鉴定与形态学鉴定结果一致，证明了 DNA 条形码技术在鉴定寄生蜂这类小型昆虫中的准确性和广阔的应用前景。
Application of DNA barcoding in identification of parasitoids on Myzus persicae
Myzus persicae is a major pest of peach trees.To elucidate the species diversity of its parasitoids,we collected parasitized aphids from peach orchards in the coastal regions of Jiangsu.The emerged adult parasitoids were identified using a combination of morphological characteristics and DNA barcoding methods.Our survey identified five parasitoid species belonging to two families in this region:Aphelinus abdominalis and Aphelinus maculatus from the family Aphelinidae;and Lipolexis gracilis,Binodoxys communis,and Aphidius gifuensis from the family Braconidae.The COI gene sequences of these five parasitoid species were extracted and analyzed by constructing a phylogenetic tree.The molecular identification results were consistent with the morphological identification results,demonstrating the accuracy and extensive application potential of DNA barcoding technology in identifying small insects such as parasitoids.
DNA barcodingparasitoidsCOI geneidentificationbiological controlMyzus persicae
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