Cloning and bioinformatics analysis of 5'UTR of bovine FMR1 gene
To explore the biological function of fragile X mental retardation 1(FMR1)in Simmental cattle and to provide data support for the study of the role of FMR1 in the infertility of Simmental cattle,in this study,the complete 5'UTR sequence of FMR1 gene was cloned by PCR using Simmental cattle blood DNA as a template.It was compared with the full mRNA sequence of bovine FMR1 gene provided by NCBI.Online software was used for genetic analysis and phylogenetic tree construction to analyze the fragility of the 5'UTR end of FMR1 gene.The physicochemical properties,hydrophilicity,phosphorylation sites,transmembrane structure,signal peptide,secondary structure,tertiary structure and interacting proteins of the protein encoded by this gene were analyzed.The results showed that the 5'UTR sequence of Simmental cattle FMR1 gene was basically consistent with the sequence of cattle FMR1 gene provided by NCBI,and the longest open reading frame of bovine FMR1 gene was 1 710 bp,the gene encoded 569 amino acids,and the molecular weight was 64 221.90 u,and the encoded protein was an unstable hydrophilic protein,with no obvious signal peptide cleavage site,and did not include the transmembrane helix,and the secondary structure prediction indicated that it was mainly in α-helix and random coil form.The results indicated that the coding sequence of Simmental cattle FMR1 gene was 40 254 bp and encoded 569 amino acids,and the encoded protein was a hydrophilic unstable protein,which showed the closest genetic distance from sheep.
Simmental cattleFMR1 genecloningbioinformatics analysisphylogenetic tree
NETL
NSTL
万方数据
