牛FMR1基因5'UTR克隆及生物信息学分析
Cloning and bioinformatics analysis of 5'UTR of bovine FMR1 gene
王选艺 1孙亚伟 1王俪颖 1龙雨薇 1叶彤 1周瑜新 1马雪连 1李娜 1赵红琼 1姚刚1
作者信息
- 1. 新疆农业大学 动物医学学院,新疆 乌鲁木齐 830052
- 折叠
摘要
为了探讨西门塔尔牛脆性 X智力低下基因 1(fragile X mental retardation 1,FMR1)的生物学功能,并为研究 FMR1 在西门塔尔牛屡配不孕中发挥的作用提供数据支持.实验以西门塔尔牛血液 DNA 为模板,通过PCR方法克隆FMR1 基因完整的 5'UTR序列;与NCBI提供的牛FMR1 基因mRNA全序列进行比对;利用在线软件进行遗传分析并构建系统进化树,分析 FMR1 基因 5'UTR 端脆性;分析该基因编码蛋白的理化性质、亲疏水性、磷酸化位点、跨膜结构、信号肽、二级结构、三级结构和互作蛋白.结果表明:西门塔尔牛 FMR1 基因 5'UTR序列与 NCBI提供的牛 FMR1 基因序列基本一致,生物信息学分析得到牛 FMR1 基因最长开放阅读框 1 710 bp,基因共编码 569 个氨基酸,分子量 64 221.90 u,其编码蛋白是不稳定的亲水蛋白,没有明显的信号肽切割位点,并且不包括跨膜螺旋,二级结构预测表示其主要以 α 螺旋和无规卷曲形式为主.说明西门塔尔牛 FMR1 基因编码序列长度为 40 254 bp,编码 569 个氨基酸,编码的蛋白质为亲水不稳定蛋白,与绵羊遗传距离最近.
Abstract
To explore the biological function of fragile X mental retardation 1(FMR1)in Simmental cattle and to provide data support for the study of the role of FMR1 in the infertility of Simmental cattle,in this study,the complete 5'UTR sequence of FMR1 gene was cloned by PCR using Simmental cattle blood DNA as a template.It was compared with the full mRNA sequence of bovine FMR1 gene provided by NCBI.Online software was used for genetic analysis and phylogenetic tree construction to analyze the fragility of the 5'UTR end of FMR1 gene.The physicochemical properties,hydrophilicity,phosphorylation sites,transmembrane structure,signal peptide,secondary structure,tertiary structure and interacting proteins of the protein encoded by this gene were analyzed.The results showed that the 5'UTR sequence of Simmental cattle FMR1 gene was basically consistent with the sequence of cattle FMR1 gene provided by NCBI,and the longest open reading frame of bovine FMR1 gene was 1 710 bp,the gene encoded 569 amino acids,and the molecular weight was 64 221.90 u,and the encoded protein was an unstable hydrophilic protein,with no obvious signal peptide cleavage site,and did not include the transmembrane helix,and the secondary structure prediction indicated that it was mainly in α-helix and random coil form.The results indicated that the coding sequence of Simmental cattle FMR1 gene was 40 254 bp and encoded 569 amino acids,and the encoded protein was a hydrophilic unstable protein,which showed the closest genetic distance from sheep.
关键词
西门塔尔牛/FMR1基因/克隆/生物信息学分析/系统进化树Key words
Simmental cattle/FMR1 gene/cloning/bioinformatics analysis/phylogenetic tree引用本文复制引用
基金项目
新疆维吾尔自治区科技成果转化示范专项乡村振兴产业发展科技行动项目(2022NC098)
出版年
2024
NETL
NSTL
万方数据