A mechanical centrifugal technique was proposed to clean the agar in the tissue culture containers because of the difficulty of automatic cleaning due to the agar sticking to the wall of the cups.The effects of the rotation speed of the scraper,the rotation time of the scraper,the size of the scraper and the way of scraping in and out of the bottle on the cleaning effect of the containers by a one-way test.The orthogonal test was used to obtain the optimal combination of test conditions,and the results showed that the scraper rotation speed was 210 r·min-1,the scraper rotation time was 2.5 s,the scraper in/out mode was positive-reverse rotation,the scraper radius was 28 mm,the effect of agar clearing of the container in the tissue culture reached more than 95%,which can meet the actual production requirements of the tissue culture container cleaning and filling.The test results provide technical support for the research and development of automatic cleaning and filling equipment for recycling-type tissue culture containers,and have the significance of engineering promotion.
维普
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