Effects of plant growth regulators on seed germination and stress resistance of tobacco seedlings
In order to explore the effects of plant growth regulators on tobacco seed germination and seedling growth,the effects of 10 plant growth regulators on the germination of tobacco seeds and the growth of tobacco seedlings and their optimal concentrations were determined by two-factor analysis.The germination test showed that the germination rate of tobacco seeds treated with 1.6 mg·L-1 gibberellin(GA3)and 750 times diluted compound humic acid were the best,and the germination rate of tobacco seeds were 100.00%,which were 30.00 percentage points higher than that of the control.The results of floating seedling test showed that 0.4 mg·L-1 indoleacetic acid(IAA),1.6 mg·L-1 β-naphthoxyacetic acid and 750 times diluted compound humic acid had good promoting effects on the growth of floating tobacco seedling.The results of pot experiment showed that the fresh weight,plant height,main root length and maximum leaf area of potted tobacco treated with 1.6 mg·L-1 GA3 and 0.4 mg·L-1 IAA increased to varying degrees.When transplanted for 60 days,the fresh weight,plant height,main root length,maximum leaf area and chlorophyll content of potted tobacco treated with 1.6 mg·L-1 GA3 increased by 34.06%,44.71%,29.25%,54.03%and 16.85%,respectively,compared with the control.At the same time,after the treatment of plant growth regulators,the catalase(CAT)activity and malondialdehyde(MDA)content in the roots of floating tobacco seedling and potted tobacco decreased,and stress resistance of tobacco seedlings were improved.In summary,1.6 mg·L-1 GA3,0.4 mg·L-1 IAA and 750 times diluted compound humic acid can effectively promote tobacco seed germination and seedling growth.
万方数据
维普
