Mutation screening and identification of wide-compatibility restorer rice line resistant to imidazolinone herbicide
Breeding for rice varieties with resistance to imidazolinone herbicides is one of the most economical and effective strategies to control weeds in rice fields.Imidazolinone herbicides target acetyllactate synthase and can control the vast majority of common weeds in rice fields.In this study,we screened 12 rice materials resistant to imidazolinone herbicides by inducing the wide-compatibility restorer line DR610 with 350 Gy 60Co-γ radiation and EMS mutagens.Through pairing with the three japonica male sterile lines J14A,Changgeng 1A,and Zhegeng 7A,three wide-compatibility restoring lines were ultimately screened.Furthermore,sequence analysis showed that G/A mutation at position 1 880 of ALS gene,which led to 627th amino acid alteration from serine to asparagine,conferring herbicide resistance to three wide-compatibility restoring lines.This study was the first to screen herbicide-resistant wide-compatibility restorer lines by double mutagenesis with a practical potential in breeding for new herbicide-resistant indica-japonica hybrid rice varieties.
万方数据
维普
