Comparison of three nucleic acid screening systems in the detection of blood donors in Wenzhou
Objective This study aims to evaluate and compare the efficacy of Cobas s201,Panther and ChiTaS BSS 1200/PreNAT II's nucleic acid testing(NAT)systems in detecting blood donations in Wenzhou.Methods This study includ-ed 586970 blood samples collected from blood donors in Zhejiang Province,Wenzhou from January 2018 to May 2023,and detected by Cobas s201,Panther and ChiTaS BSS1200/PreNAT II nucleic acid detection systems.The three systems adopt different detection modes:the Cobas s201 system adopts a 6-sample detection mode;the Panther system adopts a single detection mode;and the ChiTaS BSS1200/PreNAT II system adopts an 8-sample/single detection mode.Results The posi-tive detection rates of the three systems of Cobas s201,Panther and ChiTaS BSS1200/PreNAT II are:0.90‰(344/381919),3.54‰(440/124303)and 2.59‰(209/80748),and the positive detection rate of Panther is significantly higher,Compared with the other two systems(P<0.05);NAT-positive cases in Wenzhou area were mainly due to HBV infection,and the infec-tion rate was 1.22‰(717/586970);among the 42 HBV-positive specimens tested by NAT,they were mainly occult infec-tions.Conclusion The results of this study show that the three nucleic acid screening systems,Cobas s201,Panther and ChiTaS BSS1200/PreNAT II,can effectively detect nucleic acid-positive samples in enzyme immunoassay non-reactive blood samples from voluntary blood donors in Wenzhou,thereby reducing missed detections in ELISA testing.The Panther system showed a high positive detection rate,demonstrating its testing superiority in the region.In addition,NAT testing has a sig-nificant role in detecting occult HBV infection,which is of great significance in preventing transfusion-transmitted diseases.Therefore,regular analysis of test data,combined with the rational application of NAT detection systems,can further reduce the risk of transfusion-related infectious diseases.
万方数据
维普
