A novel dual-component crosslinked encapsulated polymer hydrogel promoting dermal wound healing in rat
Objective To explore the impact of a novel dual-component crosslinked encapsulated polymer hydrogel in wound healing of rats.Methods A wound model was developed by piercing the dorsal skin in SD rats.The rats were then randomly divided into three groups,namely the hydrogel group,gentamycin sulfate group and control group,with 6 rats in each group.The hydrogel group was treated daily with 25 μL aqueous solution of plant cellulose derivative on the wound site,along with the application of 25 μL oxidized starch solution.The gentamicin sulfate group was treated with 50 μL gentamycin sulfate on the wound site every day.The control group received no treatment.The wound healing conditions were observed and the wound surface area was measured during drug intervention.After 10 days of drug intervention,the rats were anesthetized and the area of granulation tissue in the wound site was measured,and the relative expression levels of IL-6,TNF-α and transforming growth factor β(TGF-β)mRNA in the tissues were detected.Results From the fourth day of drug intervention,the wound surface area of rats in the hydrogel group and the gentamicin sulfate group was both significantly smaller than that of the control group(both P＜0.05).However,there was no statistical significance in wound surface area between the hydrogel group and the gentamycin sulfate group at all time-points(all P＞0.05).In the hydrogel and gentamycin sulfate groups,areas of the granulation tissues were both significantly smaller than those of the control group,the expression levels of IL-6 and TNF-α mRNA in the tissues were significantly lower,and TGF-β mRNA expression level was significantly higher(all P＜0.05).No statistically significant differences in area of wound granulation tissues,and relative expression levels of IL-6,TNF-α and TGF-β mRNA were observed between the hydrogel group and the gentamicin sulfate group(all P＞0.05).Conclusion This new dual-component crosslinked encapsulated polymer hydrogel accelerates wound healing and reduces inflammation of rats.
万方数据
维普
