Differential expression of exosome protein and its pathogenesis in patients with inflammatory bowel disease complicated with skin lesions
Objective To investigate the differential expression of exosome protein and its pathogenesis in patients with inflammatory bowel disease(IBD)complicated with skin lesions.Methods Five patients with IBD and another five patients with IBD combined with skin damage admitted to Hangzhou Third People's Hospital from January 2020 to December 2021 were selected as the study objects as respectively the IBD group and IBD+skin damage group.In addition,five age-and sex-matched healthy volunteers were selected as the control group.Serum exosomes were extracted from patients in the 3 groups and their differentially expressed proteins were identified.Bioinformatic analysis of differentially expressed proteins was performed using gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG).Results According to proteomic results,a total of 829 proteins were identified,including 726 proteins with quantitative information.Compared with the control group,15 proteins were up-regulated and 21 proteins were down-regulated in IBD group,while in IBD+skin damage group,24 proteins were up-regulated and 25 proteins were down-regulated.Compared with IBD group,12 proteins were up-regulated and 8 proteins were down-regulated in IBD+skin damage group.GO analysis showed that the biological processes involved in the differential expression of proteins between the IBD group and the control group,between the IBD+skin damage group and the control group,and between the IBD+skin damage group and the IBD group included stimulation response,cellular processes,single organic processes,biological regulation,etc.The cell composition mainly included cells,outer membrane domain,organelles,etc.The molecular functions mainly included binding and catalytic activity.KEGG analysis showed that the difference of protein expression between IBD group and control group was mainly related to cell adhesion,ion transmembrane transport and cell morphological differentiation.The difference of expression of protein between IBD+skin damage group and control group was mainly related to cell secretion,cell catabolism and secretion regulation.The difference of protein expression between IBD+skin damage group and IBD group was mainly related to negative regulation of cellular amine metabolism,NIK/NF-κB signaling pathway,and cell cycle processes.Conclusion In this study,based on TMT proteomic analysis,differential expression proteins of serum exosomes in patients with IBD+skin damage are screened,and these proteins are involved in metabolic pathways and biological functions associated with IBD and skin damage.
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