Effects of curcumin on hippocampus synaptic remodeling of epileptic rats by regulating miR-134
Objective To investigate the mechanism of curcumin exerting neuroprotective and potential antiepileptic effects through modulation of miR-134.Methods Forty-eight rats were divided into three groups of control,epilepsy and curcumin according to the random number table method,with 16 rats in each group.Except for the control group,the remaining two groups applied lithium chloride-pilocarpine to induce rat models of status epilepticus.After modeling,rats in the curcumin group were intraperitoneally injected with 2 mL/kg of curcumin,while rats in the epilepsy group and the control group were intraperitoneally injected with 2 mL/kg of dimethylsulfoxide,both at a fixed time every day from day 2,and the drugs were administered continuously for 10 d.Rats were sacrificed by cervical dislocation on the 14th day after successful modeling,and the degree of mossy fiber sprouting in the hippocampal tissue of rats was observed by Timm staining.The expression levels of miR-134 in rat hippocampal tissue and serum were detected by qRT-PCR,and the protein expression level of LIM kinase 1(LIMK1)in rat hippocampal tissue was detected by Western blot.Results Compared with the control group,the scores of the degree of mossy fiber outgrowth in the dentate gyrus and CA3 region of rats in the epilepsy group were both elevated(both P＜0.05);while compared with the epilepsy group,those scores in the curcumin group were decreased(both P＜0.05).Compared with the control group,the expression level of miR-134 in the hippocampal tissue of rats in the epilepsy group was increased(P＜0.05),while the expression level of LIMK1 protein was decreased(P＜0.05);Compared with the epilepsy group,the miR-134 level in the hippocampal tissue of rats in the curcumin group was decreased(P＜0.05),while LIMK1 protein expression level was increased(P＜0.05).Compared with the control group,serum miR-134 expression levels of rats in the epilepsy and curcumin groups were both decreased(both P＜0.05).Conclusion Curcumin inhibits miR-134 expression and upregulates LIMK1 levels in hippocampal tissues after sustained epileptic status in rats,affecting synaptic remodeling,and may thus exert neuroprotective and potentially antiepileptic effects.
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