Identification,distribution and drug resistance of 308 strains of nontuberculous Mycobacterium
Objective To identify and analyze the distribution trend and drug resistance of clinical isolated nontuberculous Mycobacteria(NTM).Methods Clinical samples of sputum,alveolar lavage fluid,wound secretion,lung puncture tissue,and pleural effusion were collected from 18 006 patients admitted to Yueqing People's Hospital from January 2018 to December 2022.The samples were cultured,the bacterial strains were identified,and the drug susceptibility was tested by broth microdilution method.Results A total of 308 strains of NTM were isolated,of which 191 were isolated from sputum samples accounting for 62.1％,and 105 were isolated from bronchoalveolar lavage fluid accounting for 34.1％.Among the 308 patients,209 strains(67.9％)were from female patients,and 99 strains(32.1％)were from male patients;and from patients aged 50-70 years accounted for 52.9％(n=163).A total of 19 kinds of NTM were detected,of which 184(59.7％)were Mycobacterium intracellulare,45(14.6％)were Mycobacterium abscessus,and 17(5.5％)were Mycobacterium av-ium.Mycobacterium intracellulare strains were highly sensitive to clarithromycin and amikacin with a sensitivity rate of 95.6％.However,the resistance to linezolid and moxifloxacin was very high with a resistance rate higher than 50.0％.The resistance rate of Mycobacterium avium to linezolid was high,reaching 69.2％.The susceptibility rates of slow-growing NTM(except Mycob-acterium Avium-Intracellulare complex)to clarithromycin,rifampicin,amikacin,and rifabutin were higher than 78.0％.The sensitivity of Mycobacterium abscessusto amikacin and clarithromycin was 100.0％and 70.0％,respectively;the resistance rates to doxycycline,moxifloxacin,ciprofloxacin,and trimethoprim-sulfamethoxazole were 100.0％;the resistance rate to imipenem and tobramycin was 70.0％.For other fast-growing nontuberculous mycobacteria,the rates of resistance to imipenem,tobramycin,doxycycline,ciprofloxacin,and trimethoprim-sulfamethoxazole were more than 80.0％.Conclusion The NTM isolated in this study are mainly Mycobacterium intracellulare,Mycobacterium abscessus,and Mycobacterium avium,which are highly sensitive to amikacin and clarithromycin and highly resistant to doxycycline,which provided support for impnving the precision diagnosis and treatment level of NTM in Yueqing area.
Nontuberculous MycobacteriaIdentification of speciesDistribution of speciesAnalysis of drug resistance
万方数据
维普
