Protective effect and mechanism of Anoectochilus roxburghii extract on acute lung injury induced by LPS in mice
Objective To investigate the effects of Jinxianlian(JXL,Anoectochilus roxburghii)extract on lipopoly-saccharide(LPS)-induced acute lung injury in mice and explore its mechanism.Methods Sixty healthy ICR mice were ran-domly divided into control group(CON),model group(LPS),dexamethasone(DEX)group,low-dose JXL extract(JXL-L)group,medium-dose JXL extract(JXL-M)group,high-dose JXL extract(JXL-H)group.DEX solution(5.0 mg/kg)was given by intraperitoneal injection in DEX group,different doses of JXL extract solution(1.3,2.6,5.2 mg/kg)were given by gavage in the JXL-L,JXL-M,and JXL-H groups,while the blank group and model group were given normal saline by gavage for 2 d.On the D3,mice in the normal group were instilled with normal saline through the nose,and the other groups were instilled with LPS(5.0 mg/kg)to induce acute lung injury(ALI).Twelve hours after the last administration,mice were sacrificed,and lung tissue and bronchoalceolar lavage fluid(BALF)samples were collected.The lung wit/dry weight ratio(W/D)was measured,and HE staining was used to observe the pathological changes of lung tissue.The levels of IL-6,monocyte chemoattractant protein-1(MCP-1),IL-12/p70,IFN-γ,TNF-α and IL-10 in BALF were measured by ELISA.The relative expression of myeloperoxidase(MPO),phosphorylated activated extracellular signal-regulated kinase(p-ERK),nuclear factor kappa-B(IκBα)and phosphorylated IκBα(p-IκBα)in lung tissue were measured by immunohistochemistry(IHC).The expression of related proteins ERK,p-ERK,c-Jun N-terminal kinase(JNK),phosphorylated JNK(p-JNK),p38,phosphorylated p38(p-p38),adenosine monophosphate actived protein kinase α(AMPKα),phosphorylated AMPKα(p-AMPKα),MPO in lung tissue was detected by Western blot.Results Compared with the control group,the model group displayed inflammatory pathological changes in lung tissue,and the levels of IL-6,MCP-1,IL-12/p70,IFN-γ,TNF-α and IL-10 in BALF were increased(all P＜0.01).Compared with the model group,the pathological changes of the JXL-H group were attenuated,lung W/D,and the levels of IL-6,MCP-1,IL-12/p70,TNF-α,IL-10 in BALF were decreased,the relative expression of MPO,p-ERK and p-IκBα were decreased,while the expression of related proteins of p-ERK,p-JNK,p-p38,p-AMPKα and MPO in lung tissue were downregulated too(all P＜0.01).Conclusion JXL extract with certein dose has a protective effect against LPS-induced acute lung injury in mice,which may be related to its inhibition of mitogen-activated protein kinase signaling pathway and the improvement of inflammatory response.
Anoectochilus roxburghiiAcute lung injuryMitogen-activated protein kinase signaling pathwayInflammatory factors
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