Study on the effect of functionalized sodium alginate hydrogel on proliferation,condensation and chondrogenic differentiation of bone marrow mesenchymal stem cells
Objective To investigate whether RGD polypeptide-modified acylated sodium alginate hydrogel matrix can regulate the behavioral activity of bone marrow mesenchymal stem cells(BMSCs)and promote their chondrogenic differentiation.Methods RGD polypeptide was grafted to acylated sodium alginate by Michael addition reaction.The double bonds of the photoinitiator and the acylated sodium alginate were cross-linked to gel under ultraviolet light irradiation.The effects of functional hydrogels on proliferation,aggregation and chondrogenic differentiation of BMSCs were evaluated by CCK-8 assay,scanning electron microscopy(SEM),cytoskeleton staining,immunofluorescence staining,Western Blot(WB)and RT-PCR.Results Compared with the ungrafted group,RGD polypeptide-modified acylated sodium alginate hydrogel could promote cell morphology extension and aggregation in the matrix.After RGD polypeptide grafting,the proliferation activity of cells was significantly higher than that of the non-grafted group in two or three dimensions.After chondrogenic induction culture,RGD polypeptide-modified acylated sodium alginate hydrogel could better promote cell aggregation and chondrogenic differentiation.Conclusion The acylated sodium alginate hydrogel grafted with RGD polypeptide can better promote the proliferation,aggregation and chondrogenic differentiation of BMSCs.
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