Effect of berberine on inhibiting multi-drug resistance of Helicobacter pylori
Objective To study the effect of berberine on inhibiting Helicobacter pylori(Hp)multi-drug resistant(MDR)strains that are resistant to common antibiotics.Methods E-test was used to detect the drug resistance of clinical isolates,and double-drug resistant(DR)strains and MDR strains were screened.Then the DR strains were induced to MDR strains in vitro by two-fold increasing of antibiotic concentration method.The minimum inhibitory concentration(MIC)of berberine against MDR strains was detected by agar two-fold dilution method.The MDR strains were cultured continuously at 1/2 MIC concentration of berberine in liquid,and the MIC values of corresponding antibiotics were detected by E-test after one and five generations,respectively.Results(1)One hundred and twenty-eight Hp strains were isolated,including thirty DR strains and twelve MDR strains according to the drug susceptibility test.The drug resistance rates of Hp to amoxicillin(AMX),levofloxacin(LEV),clarithromycin(CLR)and metronidazole(MTZ)were 4.7％,26.6％,32.0％and 64.8％,and MDR rate in isolated strains was 9.4％.(2)There were 24 MDR strains induced in vitro successfully.Strains induced by AMX were failed.The resistance level of induced strains by MTZ was the highest,followed by those of CLR and LEV.(3)Berberine could significantly inhibit the growth of the 36 MDR strains,MIC values of which vary from 8 μg/mL to 128 μg/mL,mostly at 32 µg/mL.(4)Of the 27 MDR strains selected with high-level resistance,the MIC values of 17 strains were reduced by more than four times or reversed into sensitive strains after berberine treatment,among which,9,11 and 5 strains showed positive changes in MIC values corresponding to CLR,LEV and MTZ,respectively.Among the positive strains of three antibiotics,the median decline multiple of MIC value was the highest in MTZ group,followed by CLR group and LEV group,and the difference among the three groups was statistically significant(P＜0.05).Conclusion Berberine can effectively reverse resistance of some MDR strains to common antibiotics,and the degree of reversal is related to the action time and antibiotic type.
万方数据
维普
