Effects of low-protein and keto acid diet intervention on kidney functions of mice with diabetic nephropathy
Objective To explore the effects of low-protein diet and keto acid supplementation on renal tissue structure and function in mice with diabetic nephropathy.Methods Eighteen male db/db mice were randomly assigned to the db/db group(normal-protein diet group),db/db+low-protein diet group(low-protein diet group),and db/db+low protein-keto acid diet group(low protein-keto acid diet group),with 6 in each group.Six db/m mice were included in the db/m group(normal control group),which were given normal-protein diet.Dietary intervention lasted for 12 weeks,and the body weight of mice was measured every 2 weeks.After feeding,the mice fasted overnight that day,and on the next day,the 24 h water intake and urine output of mice were recorded,after which,blood glucose,creatinine,urea nitrogen,TG,and TC of the mice were measured;mice were then sacrificed,and their kidneys were collected to calculate the weight,and measure the transverse diameter to roughly calculate the kidney volume,and calculate the ratio of kidney weight to body weight;HE and PAS staining were used to observe the renal tissue mesangial cell proliferation,and imunohistochemical staining were used to evaluate the expressions of transforming growth factor(TGF)-β1 and collagen type Ⅳ in kidney tissues.Results Compared with normal control group,the mice of normal-protein diet group showed increased body weight,water intake and urine output(all P＜0.05),as well as elevated levels of blood glucose,creatinine,urea nitrogen,TG,and TC(all P＜0.05);the tissues around kidneys increased in fat and volume,and the staining results showed increased kidney tissue mesangial cell proliferation,and TGF-β1 and collagen type Ⅳ expressions(all P＜0.05).Compared with normal-protein diet group,the mice of low-protein diet group and low protein-keto acid diet group displayed decreased body weight,reduced water intake and urine output(all P＜0.05),as well as reduced levels of blood glucose,creatinine,urea nitrogen,TG,and TC(all P＜0.05);the tissues around kindeys decreased in fat and volume,and the staining results showed less kidney tissue mesangial cell proliferation,and decreased TGF-β1 and collagen type Ⅳ expressions(all P＜0.05).Conclusion Low-protein diet combined keto acids could improve renal function of diabetic nephropathy mice by improving biochemical indicators and reducing renal tissue TGF-β1 and collagen type Ⅳ expressions.
万方数据
维普
