首页|脑卒中后认知与非认知功能障碍患者肠道菌群特征的差异研究

脑卒中后认知与非认知功能障碍患者肠道菌群特征的差异研究

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目的 比较脑卒中后认知功能障碍(PSCI)与脑卒中后非认知功能障碍(non-PSCI)患者肠道菌群特征的差异.方法 对2022年2月至2023年2月嘉兴市第二医院康复中心收治的PSCI患者(观察组)及性别、年龄配对的non-PSCI患者(对照组)采用简易精神状态检查量表(MMSE)并根据患者的文化水平进行调整,各44例,收集其粪便样本,采用16SrRNA扩增子测序获得肠道菌群信息,对两组样本行α多样性分析和β多样性分析,同时在门和属分类层级对样本进行注释以及汇总计算相对丰度,比较两组患者门水平Top10和属水平Top30的肠道菌群.结果 两组患者粪便肠道菌群α多样性的差异均无统计学意义(均P>0.05),β多样性的差异有统计学意义(P=0.048).观察组中Bifidobacterium和Lachnospiraceae_NK4A136_group相对丰度分别为 0.05(0.01,0.28)和0.03(0,0.31),低于对照组0.17(0.04,1.78)和 0.18(0.03,0.81),Enterobacter、Lachnoclostridium 和UBA1819相对丰度分别为 0.08(0,0.41)、0.97(0.39,2.59)和 0.20(0.07,1.35),高于对照组0.01(0,0.16)、0.42(0.19,0.85)和0.09(0.02,0.31),差异均有统计学意义(均P<0.05).结论 PSCI患者与non-PSCI患者在肠道菌群结构特征上存在差异,Bifi-dobacteriu和Lachnospiraceae_NK4A136_group可能可以作为潜在的益生菌辅助PSCI治疗.
Characteristics differences of gut microbiota between post-stroke patients with or without cognitive impairment
Objective To compare characteristic differences of gut microbiota between post-stroke cognitive impairment(PSCI)and non-post-stroke cognitive impairment(non-PSCI)patients.Methods The mini-mental state examination(MMSE)scale,adjusted based on patient education level,was used to select PSCI patients(observation group)and age and gender-matched non-PSCI patients(control group)from the Rehabilitation Medicine Center of the Second Hospital of Jiaxing from February 2022 to February 2023,each with 44 cases.Fecal samples were collected from all participants,and 16S rRNA amplicon sequencing was performed to obtain gut microbiota information.α diversity and βdiversity analyses were conducted for the two groups of samples.Samples were annotated and summarized at the phylum and genus levels,and relative abundance was calculated.The phylum-level Top 10 and genus-level Top 30 gut microbiota were compared between the two groups.Results There were no significant differences in α diversity between the two groups(all P>0.05),but a significant difference in β diversity was observed(P=0.048).The relative abundance of Bifidobacterium and Lachnospiraceae_NK4A136_group in the observation group was 0.05(0.01,0.28)and 0.03(0,0.31),respectively,which were lower than that of the control group of 0.17(0.04,1.78)and 0.18(0.03,0.81).The relative abundance of Enterobacter,Lachnoclostridium,and UBA1819 in the observation group was 0.08(0,0.41),0.97(0.39,2.59),and 0.20(0.07,1.35),respectively,which were higher than that of the control group of 0.01(0,0.16),0.42(0.19,0.85),and 0.09(0.02,0.31).All these differences were statistically significant(all P<0.05).Conclusion There are differences in the structural characteristics of gut microbiota between PSCI and non-PSCI patients.Bifidobacterium and Lachnospiraceae_NK4A136_group may be used as potential probiotics to assist in the treatment of PSCI.

Post-stroke cognitive impairmentCognitive functionGut microbiotaProbiotics

王中莉、曾明、任芸、沈雅萍、孙燕、施明、顾旭东、傅建明、姚云海

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314000 嘉兴市第二医院康复医学中心

脑卒中后认知功能障碍 认知功能 肠道菌群 益生菌

浙江省医药卫生科技计划项目临床研究应用项目浙江省医药卫生科技计划项目临床研究应用项目浙江省医药卫生科技计划项目临床研究应用项目

2022KY3832022KY3782022KY1252

2024

浙江医学
浙江省医学会

浙江医学

CSTPCD
影响因子:0.428
ISSN:1006-2785
年,卷(期):2024.46(6)
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