Characteristics differences of gut microbiota between post-stroke patients with or without cognitive impairment
Objective To compare characteristic differences of gut microbiota between post-stroke cognitive impairment(PSCI)and non-post-stroke cognitive impairment(non-PSCI)patients.Methods The mini-mental state examination(MMSE)scale,adjusted based on patient education level,was used to select PSCI patients(observation group)and age and gender-matched non-PSCI patients(control group)from the Rehabilitation Medicine Center of the Second Hospital of Jiaxing from February 2022 to February 2023,each with 44 cases.Fecal samples were collected from all participants,and 16S rRNA amplicon sequencing was performed to obtain gut microbiota information.α diversity and βdiversity analyses were conducted for the two groups of samples.Samples were annotated and summarized at the phylum and genus levels,and relative abundance was calculated.The phylum-level Top 10 and genus-level Top 30 gut microbiota were compared between the two groups.Results There were no significant differences in α diversity between the two groups(all P>0.05),but a significant difference in β diversity was observed(P=0.048).The relative abundance of Bifidobacterium and Lachnospiraceae_NK4A136_group in the observation group was 0.05(0.01,0.28)and 0.03(0,0.31),respectively,which were lower than that of the control group of 0.17(0.04,1.78)and 0.18(0.03,0.81).The relative abundance of Enterobacter,Lachnoclostridium,and UBA1819 in the observation group was 0.08(0,0.41),0.97(0.39,2.59),and 0.20(0.07,1.35),respectively,which were higher than that of the control group of 0.01(0,0.16),0.42(0.19,0.85),and 0.09(0.02,0.31).All these differences were statistically significant(all P<0.05).Conclusion There are differences in the structural characteristics of gut microbiota between PSCI and non-PSCI patients.Bifidobacterium and Lachnospiraceae_NK4A136_group may be used as potential probiotics to assist in the treatment of PSCI.