Sequential use of vascular endothelial growth factor and endothelial progenitor cells in improving liver ischemia-reperfusion in-jury
Objective To investigate the role of sequential use of vascular endothelial growth factor(VEGF)and endothelial progenitor cells(EPC)in improving liver ischemia reperfusion injury(IRI).Methods Eight healthy Wistar rats were selected for isolation,cultivation,and identification of bone marrow EPC.A total of 28 healthy Wistar rats were selected to establish a liver IRI rat model,which was randomly divided into VEGF+EPC group,VEGF group,EPC group,and surgical control group,with 7 rats in each group.Drugs were injected through the mesenteric vein branch.The VEGF+EPC group was sequentially injected with recombinant rat VEGF factor and EPC cell suspension,the VEGF group was injected with recombinant rat VEGF factor and PBS solution,the EPC group was injected with PBS solution and EPC cell suspension,and the surgical control group was only injected with an equal volume of PBS solution.The survival rate,liver function,blood VEGF,liver tissue myeloperoxidase(MPO)levels,and liver tissue pathological changes of each group were compared.Results The positive rates of CD133,CD34,and the vascular endothelial growth factor receptor-2 in the isolated adherent cells were 90.51％,93.23％,and 93.41％,respectively,indicating the successful isolation of bone marrow EPC cells.There was no statistical difference in the survival rate of rats in each group(P＞0.05).After one week,the levels of ALT,lactate dehydrogenase(LDH),and TBil in the VEGF+EPC group rats were significantly lower than those in the VEGF and EPC groups(all P＜0.01),the level of VEGF was significantly higher than that of VEGF group and EPC group(all P＜0.01),and the level of MPO in liver tissues of VEGF+EPC group was significantly lower than that of VEGF and EPC groups(all P＜0.01).Pathological examination of liver tissues showed mild edema of liver cells and normal liver cord structure in the VEGF+EPC group after one week of intervention,without significant pathological changes such as inflammatory cell aggregation and liver cell necrosis.Conclusion Sequential use of VEGF and EPC in liver IRI can not improve the survival rate of rats,but can improve liver function and alleviate liver tissue inflammation,which can aid in the treatment of liver IRI.
万方数据
维普
