Expression of STAT1 in non-small cell lung cancer and its influence on the anti-tumor effect of IL-2
Objective To explore the expression of signal transducer and activator of transcription 1(STAT1)in non-small cell lung cancer(NSCLC)and its influence on the anti-tumor effect of interleukin-2(IL-2).Methods Lung cancer tissue and normal lung tissues which more than 5 cm away from the tumor tissues were collected from 20 NSCLC patients admitted in Lishui Central Hospital From January 2018 to January 2020,and the expression of STAT1 in both samples were detected by Western blot.NSCLC SPC-A-1 cells were transfected with Lenti-STAT1 or Lenti-enhanced green fluorescent protein(EGFP)by lentiviral vectors,and the cells were divided into the blank group,Lenti-EGFP control group and Lenti-STAT1 group.The cell clone formation rate was tested by plate cloning assay.After treatment with 0,20,100 and 500 U/mL IL-2,the cell proliferation was detected by CCK-8 assay.The migration and invasion ability of cells after treatment with 0 and 100 U/mL IL-2 was detected by Transwell assay.The percentage of cell apoptosis after treatment with 0 and 100 U/mL IL-2 was analyzed by flow cytometry.The influence of IL-2 on the anti-tumor effect of 3 groups of cells was observed.The expression levels of phospho STAT1(p-STAT1),intercellular cell adhesion molecule-1(ICAM-1)and proliferating cell nuclear antigen(PCNA)in 3 groups were detected by Western blot.Results The expression level of STAT1 protein in lung cancer tissues was lower than that in normal tissues(P＜0.001).The cell proliferation,migration and invasion ability of the Lenti-STAT1 group were lower than those of the Lenti-EGFP control group,and the percentage of cell apoptosis was higher than that of the Lenti-EGFP control group(all P＜0.05).After treatment with 20,100 and 500 U/mL IL-2,the cell proliferation of the Lenti-STAT1 group was lower than that of the Lenti-EGFP control group(all P＜0.05).After treatment with 100 U/mL IL-2,the migration and invasion ability of Lenti-STAT1 group were lower than those of Lenti-EGFP control group,and the percentage of cell apoptosis of the Lenti-STAT1 group was higher than that of the Lenti-EGFP control group(all P＜0.05).Furthermore,the expression level of p-STAT1 protein in the Lenti-STAT1 group without IL-2 treatment was higher than that of the Lenti-EGFP control group,and the expression level of ICAM-1 protein was lower than that of the Lenti-EGFP control group,and the differences were statistically significant(all P＜0.05).After treatment with 100 U/mL IL-2,the expression level of p-STAT1 protein in the Lenti-STAT1 group was higher than that of the Lenti-EGFP control group,and the expression level of and PCNA protein was lower than that of the Lenti-EGFP control group(all P＜0.05).Conclusion The expression of STAT1 is significantly down-regulated in NSCLC tissues,which can inhibit cell proliferation,migration and invasion,promote cell apoptosis,and enhance the anti-tumor effect of IL-2 in lung cancer cells.Therefore,the combination of STAT1 and IL-2 may be a viable approach for the treatment of lung cancer in the future.
Signal transducer and activator of transcription 1SPC-A-1 cellslnterleukin-2Lung cancer
万方数据
维普
