摘要
目的 探讨玄参提取物对脑缺血再灌注损伤(CRI)小鼠氧化应激及炎症反应的影响.方法 将60只SPF级雄性C57BL/6小鼠按随机数字表法分为模型组、正常组、阳性对照组、低剂量组和高剂量组,每组各12只.模型组、阳性对照组、低剂量组和高剂量组建立小鼠CRI模型.建模前,低、高剂量组小鼠术前分别给予5、20mL/kg玄参提取物灌胃;阳性对照组给予20 mL/kg辛伐他汀灌胃;模型组和正常组术前分别给予等量0.9％氯化钠注射液灌胃,均1次/d,连续5 d.采用Bederson评分法和Longa评分法评价小鼠神经功能;采用Morris水迷宫实验测定小鼠找到平台的时间(潜伏期)和穿越平台次数;采用硫代巴比妥酸比色法测定血清丙二醛(MDA)水平,采用黄嘌呤氧化酶法测定血清超氧化歧化酶(SOD)水平;采用ELISA法测定血清IL-6、IL-8和TNF-α水平;测量小鼠脑梗死体积百分比.结果 与正常组比较,其他各组Bederson评分、Longa评分、MDA水平、IL-6、IL-8和TNF-α水平、脑梗死体积百分比均明显增高,而SOD水平明显降低(均P＜0.05);与模型组比较,阳性对照组、低剂量组与高剂量组Bederson评分、Longa评分、MDA水平、IL-6、IL-8和TNF-α水平、脑梗死体积百分比均明显降低,而SOD水平明显增高(均P＜0.05);与阳性对照组和低剂量组比较,高剂量组Bederson评分、Longa评分、脑梗死体积百分比、MDA水平、IL-6、IL-8和TNF-α水平均明显降低,而SOD水平明显增高(均P＜0.05).其他各组第1、2、3、4天潜伏期均长于正常组(均P＜0.05);阳性对照组、低剂量组与高剂量组第1、2、3、4天潜伏期均短于模型组(均P＜0.05);高剂量组第1、2、3、4天潜伏期均短于阳性对照组和低剂量组(均P＜0.05).结论 玄参提取物对脑缺血再灌注损伤小鼠有较好的保护作用,可显著改善小鼠神经功能,明显缩小小鼠脑梗死体积,其机制可能与改善氧化应激及减轻炎症反应有关.
Abstract
Objective To explore effects of scrophulariae extract on oxidative stress and inflammatory response of mice with cerebral ischemia-reperfusion injury(CRI).Methods Sixty SPF grade male C57BL/6 mice were divided into model group,normal group,positive control group,low-dose group,and high-dose group using a random number table method,with 12 mice in each group.The mouse CRI models were established for the model group,positive control group,low-dose group and high-dose group.Before modeling,mice in the low and high dose groups were given 5 mL/kg and 20 mL/kg of scrophulariae extract by gavage before surgery,respectively;the positive control group was given 20 mL/kg of simvastatin by gavage;the model group and the normal group were both given an equal amount of 0.9％sodium chloride injection by gavage before surgery,both once a day,for five consecutive days.The mouse neurological function was evaluated using the Bederson and Longa scoring methods;the Morris water maze experiment was used to determine the time for mice to find the platform(incubation period)and the number of times they crossed the platform;serum malondialdehyde(MDA)levels were measured using thiobarbituric acid colorimetric method,and serum superoxide dismutase(SOD)levels were measured using xanthine oxidase method;enzyme linked immunosorbent assay was used to measure the levels of serum IL-6,IL-8,and TNF-α;the volume of cerebral infarction of mice was also measured.Results Compared with the normal group,the Bederson score,Longa score,MDA,IL-6,IL-8 and TNF-α levels,and percentage of cerebral infarction volume in other groups were significantly increased,while SOD levels were significantly reduced(all P＜0.05);compared with the model group,the positive control group,low-dose group,and high-dose group showed significant reductions in Bederson score,Longa score,MDA,IL-6,IL-8 and TNF-α levels,as well as the percentage of cerebral infarction volume,while the SOD level increased significantly(all P＜0.05);compared with the positive control group and the low-dose group,the high-dose group showed a significant decrease in Bederson score,Longa score,stroke volume percentage,MDA,IL-6,IL-8 and TNF-αlevels,while the SOD level increased significantly(all P＜0.05).Compared with the normal group,the incubation periods on the 1,2,3 and 4 days in other groups were longer(all P＜0.05);the incubation periods on days 1,2,3 and 4 in the positive control group,low-dose group,and high-dose group were shorter than those of the model group(all P＜0.05);the incubation period of the high-dose group on days 1,2,3 and 4 were shorter than that of the positive control group and the low-dose group(all P＜0.05).Conclusion Scrophulariae extract has a good protective effect on mice with CRI,significantly improving their neurological function and reducing the volume of cerebral infarction.Its mechanism may be related to reducing oxidative stress and inflammatory response.
国家科技期刊平台
NSTL
万方数据