The value of intestinal flora testing in the diagnosis of colorectal adenomas and colorectal cancer
Objective To explore the diagnostic value of human fecal gut microbiota for colorectal adenoma(CRA)and colorectal cancer(CRC).Methods Retrospective selection of 160 patients who underwent colonoscopy at Sir Run Run Shaw Hospital affiliated with Zhejiang University School of Medicine from October 2020 to October 2022.Among them,55 cases(CRC group)were diagnosed with CRC through pathological examination after surgery,55 cases'(CRA group)colonoscopy examination revealed colorectal polyps and were later diagnosed with CRA through pathological examination after polypectomy,and 50 cases(HC group)showed no abnormalities through colonoscopy.Real-time fluorescence quantitative nucleic acid amplification(qPCR)method was used to perform relative quantitative detection of specific bacterial communities in fecal samples of study subjects before intestinal preparation.Compare the relative quantification of gut microbiota among three groups of study subjects:Bifidobacterium(Bb),Lactobacillus(Lb),Enterotoxigenic bacteroides fragilis(ETBF),Fusobacterium nucleatum(Fn),and Anaerobic Streptococcus(P anaerobius);analyze the diagnostic efficacy of quantitative gut microbiota relative to quantitative analysis for CRC and CRA.Results There was no statistically significant difference in the relative quantification of intestinal ETBF among the three study groups(P＞0.05),while there were statistically significant differences in the relative quantification of Bb,Lb,Fn,and P anaerobius(all P＜0.05).The relative quantification of Bb,Fn,and P anaerobius in the CRC group was higher than that in the CRA group and higher than that in the HC group(all P＜0.05).The relative quantification of Lb in the CRA group was higher than that in the CRC group and higher than that in the HC group(all P＜0.05).ROC curve analysis showed that the single bacterium P anaerobius had the best diagnostic efficacy for CRA and CRC.The combination of bacteria Fn+Lb+P anaerobius has the best diagnostic efficacy for CRA and CRC.The best diagnostic efficacy for CRA and CRC is achieved through Fn+Lb+P anaerobius+fecal occult blood test+carcinoembryonic antigen detection in bacterial joint laboratory tests.Conclusion The quantitative detection of intestinal microbiota P anaerobius has important value in the diagnosis of CRA and CRC.The combination of Fn+Lb+P anerobius and fecal occult blood test and carcinoembryonic antigen detection can improve the detection rates of CRA and CRC.
Intestinal floraColorectal adenomaColorectal cancerDiagnostic model
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