Cardioprotective effect of PGC-1α in cardiac arrest rats after resuscitation
Objective To investigating the cardioprotective effect of peroxisome proliferator activated receptor γ coactivator-1α(PGC-1α)in cardiac arrest rats after resuscitation.Methods Eighteen male SD rats were randomly divided into a control group and a PGC-1α group.Cardiac arrest model was induced by asphyxia in rats and basic vital signs were recorded before modelling.After successful resuscitation,the control group received 0.9％normal saline chloride injection,while the PGC-1α group received PGC-1α activator(22 g/L),both 0.25 mL for each rats;24 h later the rats were sacrificed and heart samples were harvisted for pathology examination.Cardiac troponin T(cTnT)levels were measured 30 min and 24 h after resuscitation.Blood gas analysis[pH,PCO2,PO2,base excess(BE)]was performed,and ATP content in the heart was measured with ATP test kit at the 24 h following resuscitation.Serum levels of IL-6 and TNF-α were measured by ELISA and the relative expression levels of IL-6 and TNF-α mRNA in cardiac tissure were detected by qRT-PCR.Results The myocardium in the control group showed signs of destruction,with broken muscle fibers and a significant increase in vacuolar cells 24 h after resuscitation.Compared to the control group,there was no signifant change of cTnT after 30 min of resuscitation;and 24 h after resuscitation,the PGC-1α group exhibited a significant decrease of cTnT content and PCO2,a increase of pH,PO2,BE and ATP content(all P＜0.05).Furthermore,serum levels of IL-6 and TNF-α were significantly decreased in the PGC-1α group compared to the control group;the relative expression levels of TNF-α and IL-6 mRNA in heart tissue were also significantly decreased(all P＜0.05).Conclusion The study indicates that mitoc-hondria may play a protective role in cardiac reperfusion injury due to cardiac arrest through PGC-1α signaling pathway in rats.
万方数据
维普
