Expression of PGRN in breast cancer and its relation with EGFR
Objective To investigate the expression of progranulin(PGRN)in breast cancer and its relasion with epidermal growth factor receptor(EGFR).Methods Ninety five samples of breast cancer tissue and 20 samples of non-tumorous tissue were collected in Taizhou Central Hospital from January 2015 to December 2018.The expression of PGRN was detected with immunohistochemistry in all samples,and the relationship of PGRN expression with clinical characteristics and EGFR expression of breast cancer were analyzed.The breast cancer MDA-MB-231 cells were treated with PGRN activator LPA,cell viability and invasion ability were measured by cell counting kit-8(CCK-8),wound healing and 3D invasion assays,respectively.The reversal effects of EGFR inhibitors on cell viability and invasion ability induced by LPA were also detected.Results The expression level of PGRN in breast cancer tissue were higher than that in non-tumorous tissue(P＜0.01).There was no significant difference in the expression level of PGRN in the tumor tissues with age,tumor size,histological grade,lymph node metastasis and tripe negative(all P＞0.05).The expression of PGRN was positively corrected with EGFR expression in breast cancer tissues(r=0.353,P＜0.01).CCK-8 assay showed that the EGFR inhibitor significantly reversed the survival of MDA-MB-231 cells induced by LPA treatment for 24 h,48 h and 72 h(all P＜0.05).The Wound Healing assay showed that the EGFR inhibitor significantly inhibited cell migration induced by LPA treatment for 24 h and 48 h(both P＜0.05).The 3D invasion assay showed that the EGFR inhibitor significantly inhibited cell invasion induced by LPA on d4(P＜0.05).Conclusion PGRN expression is significantly upregulated in breast cancer tissues and is correlated with EGFR expression.Inhibiting EGFR can significantly reverse the cell survival,migration,and invasion induced by PGRN activator LPA.
Breast cancerProgranulinEpidermal growth factor receptorInvasion
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维普
