Roles of macrophage migration inhibitory factor in lung injury of sepsis mice and its relationship with NLRP3 inflammatome
Objective To investigate the roles of macrophage migration inhibitory factor(MIF)in lung injury of sepsis mice and its relationship with nucleotide-binding domain leucine-rich repeat and pyrin domain-containing receptor 3(NLRP3)inflammatome.Methods Twenty wild-type(WT)mice and twenty MIF knockout(KO)mice were randomly divided into WT control group,WT lung injury group,KO control group and KO lung injury group with 10 mice in each group.The lung injury model of sepsis was prepared by cecal ligation and perforation in WT and KO groups.Animals were sacrificed 24 hours after modelling,the dry weight to wet weight(W/D)ratio of lung tissue was calculated;the pathological changes of lung tissue was observed with HE staining and lung injury score was analyzed.The serum levels of IL-1β,IL-18,Gasdermin-N terminal(GSDMD-N)were detected with ELISA;the protein expression levels of MIF,NLRP3,cysteinyl aspartate specific proteinase-1(caspase-1),IL-1 β,IL-18 and GSDMD-N in lung tissues were detected with Western blot.Results The W/D level,lung injury score,serum IL-1 β,IL-18 and GSDMD-N contents,the protein expression levels of MIF,NLRP3,cleaved caspase-1,IL-1 β,IL-18 and GSDMD-N in lung tissues of WT lung injury group were significantly higher than those of WT control group and KO injury group(all P<0.05).Conclusion MIF KO reduces lung injury of sepsis mice,which may be related to the inhibition of NLRP3 inflammasome.
Macrophage migration inhibitory factorSepsisLung injuryNod-like receptor protein 3Inflammasome