Effects of Escherichia coli and its virulence factors in semen on sperm motility of male infertility patients
Objective To investigate the effects of Escherichia coli(E.coli)and its virulence factors in semen on sperm motility of male infertility patients Methods Semen samples of 445 sterile male patients admitted to Wenzhou Hospital of Traditional Chinese Medicine from January 2022 to February 2023 were collected,and 72 non-repeating strains of E.Coli were isolated,and sperm motility was detected after incubation with normal semen for half an hour.The strains that caused significant decrease in sperm motility were classified as sperm immobilization-positive(SI-positive)group,and the other strains were classified as sperm immobilization negative(SI-negative)group.Escoli virulence genes fimH,papGⅡ,papG Ⅲ,afa/dra,cnf-Ⅰ,kpsMT Ⅱ,hlyA,fyuA and cell O antigen(O1,O2,O4,O6)were detected with PCR method,and the detection rates of these virulence genes were compared between the two groups.Results The positive rates of fimH,fyuA,kpsMTⅡ,hlyA and cnf-Ⅰ genes were 95.8％,83.3％,81.9％,45.8％and 40.3％,respectively.Forty-two of the 72 E.coli strains significantly reduced sperm motility.The detection rate of virulence gene hlyA in the SI-positive group was significantly higher than that in SI-negative group(57.1％vs.30.0％,P＜0.05).There were no significant differences in the detection rates of other virulence genes between the two groups(all P＜0.05).Conclusion The decrease of sperm motility in semen of infertile men may be related to the α-hemolysin encoding gene hlyA of E.coli.
万方数据
维普
