Detection of HLA-E gene polymorphism in Zhejiang Han Population by PCR sequence-based typing method
Objective To analyze the polymorphism of human leukocyte antigen-E (HLA-E) gene in Zhejiang Han Population by the PCR sequence-based typing (PCR-SBT) method.Methods Peripheral blood samples of 150 platelet donors in Blood Center of Zhejiang Province were collected during February to April 2024.Genomic DNA of all the samples were extracted and the full-length sequences of HLA-E gene were amplified by the PCR method,and the exons 2-4 sequences of HLA-E gene were analyzed by Sanger sequencing.The results of HLA-E genotype were assigned by the Assign SBT v4.7.1 software.Results Four genotypes and three alleles of HLA-E were detected among 150 samples by the PCR-SBT method.The frequencies of genotypes HLA-E*01:01,01:01,HLA-E*01:01,01:03,HLA-E*01:03,01:03 and HLA-E*01:03,01:12 were 14.00％,51.33％,32.67％ and 2.00％,respectively.The allelic frequencies of HLA-E*01:01,HLA-E*01:03 and HLA-E*01:12 were 39.67％,59.33％ and 1.00％,respectively.Conclusion The genotyping of HLA-E can be performed by PCR-SBT method based on the Sanger sequencing.The polymorphism of HLA-E gene in Zhejiang Han population is highly conserved and only 3 HLA-E alleles were detected.
Human leukocyte antigen-EGenotypingPolymorphismPCR sequence-based typing method
万方数据
维普
