Effect of Tongfu-Huayu-Qingyi decoction on intestinal flora of mice with acute pancreatitis
Objective To investigate the effect of Tongfu-Huayu-Qingyi (THQ) decoction on intestinal flora of acute pancreatitis (AP) mice.Methods Fifty adult specific-pathogen-free C57BL/6 mice were divided into blank control group,AP model group,THQ group,octreotide group and Dachengqi group,with 10 mice in each group.Except for the blank control group,the AP model was induced by intraperitoneal injection of hyranolin in mice of other 4 groups.After successful modeling,THQ group and Dachengqi group received THQ decoction or Dachengqi decoction 23.5 mL/(kg·d) by gavage,respectively;while octreotide group received subcutaneous injection of octreotide 10 μg/(kg·d).The mice were sacrificed after continuous administration for 3 days.Colon tissue was taken for HE staining.Fresh fecal samples were collected from cecum,16S rDNA high-throughput sequencing and bioinformatics were used to analyze the diversity and difference of intestinal flora in 5 groups of mice.Results Compared with blank control group,colon tissue in AP model group showed a large number of inflammatory cell infiltration,absence of crypts and serious tissue damage,the HE score was significantly increased (P<0.05),Shannon index,Chao1 index and Simpson index were significantly decreased (both P<0.05).Compared with AP model group,colon tissue injury and inflammatory cell infiltration of mice in THQ group were alleviated,HE score was significantly decreased (all P<0.05),Chao1 index,Shannon index and Simpson index were significantly increased (all P<0.05).The principal coordinate analysis of β diversity showed that the distance between THQ group and AP model group was far,the distance between blank control group and AP model group was far,and the distance between blank control group and THQ group,octreotide group and Dachengqi group was close.At the genus level,the abundance of Lactobacillus in AP model group was higher than that in THQ group and blank control group (all P<0.05),while the abundance of Ruminococcus and Tremillum decreased (all P<0.05).The species difference analysis showed that the dominant bacteria genera in the blank control group were Coryneobacteria and Bifidobacteria.The dominant bacteria genera in AP model group were mainly Lactobacillus and Pseudomonas.The dominant bacteria genera in THQ group were mainly Gastrococcaceae and Tremillum.The dominant bacteria genera in Dachengqi group were mainly Weirongaceae,Deferrobacteriaceae and Mucispirillum.Conclusion Tongfu-Huayu-Qingyi decoction can regulate the intestinal flora structure of AP mice,increase the abundance of beneficial bacteria such as Ruminococcus and Spirillum,and decrease the abundance of harmful bacteria such as Pseudomonas and Lactobacillus,so as to attenuate the intestinal inflammatory response of AP mice with acute pancreatitis.
万方数据
维普
