[Objective]To isolate and purify the polysaccharide extracted from C.huchouensis Tamura and study their vitro antitumor activities.[Methods]Polysaccharide from C.huchouensis, named CHTP, was separated and purified by DEAE-Cellulose 52 ion-exchange chromatograph and Sephadex G-200 gel chromatograph.The anti-tumor activities of CHTP and its purified products were studied by MTT colorimetry.Three tumor cells such as HepG2, MGC8-03 and K562 were selected to detect their inhibitory effect.[Results]A polysaccharide fraction, named CHTPⅡ-1, was harvested after purified.When the concentration of CHTP was 250～1000 mg· L- 1 or the concentration of CHTPⅡ-1 was 125～500 mg· L 1, the inhibition rate of CHTP or CHTPⅡ-1 on HepG2, MGC8-03 and K562 cells increased with the increase of the polysaccharide concentration.There were significant differences of the OD value between the polysaccharide treatments and negative control except for MGC8-03 cells treated with 62.5 mg·L-1 CHTPⅡ-1 or K562 cells treated with 125mg· L-1 CHTP tested by MTT.[Conclusion]Both CHTP and CHTPⅡ-1 can inhibit the proliferation of the three tumor cells.Moreover, the inhibition rate is positively correlated to the amount of polysaccharides in test concentration.
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