[目的]明确"白术-苍术"药对对高脂血症(hyperlipidemia,HLP)的防治作用,结合网络药理学分析,初步阐释其作用机制.[方法]采用高脂饲料喂养复制HLP大鼠模型,同时给予不同剂量的"白术-苍术"药对进行干预;实验期末比较各组大鼠血脂水平并观察肝组织脂质沉积情况.采用网络药理学预测"白术-苍术"药对治疗HLP的潜在活性成分及作用靶点,并进一步进行实验验证.[结果]"白术-苍术"药对对高脂饮食诱导的HLP具有良好的防治作用.网络药理学分析发现,"白术-苍术"所含3β-醋酸基白术酮、汉黄芩素、3β-乙酰氧基苍术酮等成分为其治疗HLP的潜在活性成分,过氧化物酶体增殖物激活受体(peroxisome proliferators-activated receptors,PPAR)信号通路相关 PPARγ、脂肪酸结合蛋白 4(fatty acid binding protein 4,FABP4)为其潜在调节通路和作用靶点.动物实验结果表明,"白术-苍术"能显著上调HLP模型大鼠PPARγ表达.[结论]"白术-苍术"药对防治高脂饮食诱导的HLP的作用与激活PPARγ信号通路有关.
Mechanism Study of"Atractylodes Macrocephala Koidz-Atractylodes Rhizoma"Drug Pair on the Prevention and Treatment of Hyperlipidemia Based on Network Pharmacology and Transcriptome Analysis
[Objective]To clarify the therapeutic effects of the"Atractylodes macrocephala Koidz-Atractylodes Rhizoma"drug pair on hyperlipidemia(HLP)and to illuminate the primary mechanism via network pharmacology analysis.[Methods]HLP rat model was established by feeding rats with high-fat diet,and different doses of"Atractylodes macrocephala Koidz-Atractylodes Rhizoma"drug pair were administrated at the same time.At the end of the experiment,comparing the blood lipid levels of each group of rats and observing the lipid deposition in liver tissue.Using network pharmacology to predict the potential active ingredients and targets of"Atractylodes macrocephala Koidz-Atractylodes Rhizoma"drug pair for treating HLP,and further experimental verification.[Results]The drug pair of"Atractylodes macrocephala Koidz-Atractylodes Rhizoma"had a good effect on anti HLP induced by high-fat diet.Network pharmacology analysis showed that 3[3-acetoatractylodes atractylodes,wogonin,and 3 β-acetoxyatractylodes atractylodes were the potential active ingredients of the drugs in the treatment of HLP,and peroxisome proliferators-activated receptors(PPAR)signaling pathway-related PPARγ and fatty acid binding protein 4(FABP4)were the potential regulatory pathways and targets.The results of the validation experiment further confirmed that the drug pair could significantly up-regulate the expression of PPARy in liver tissue of rats with HLP.[Conclusion]The effect of"Atractylodes macrocephala Koidz-Atractylodes Rhizoma"drug pair on high-fat diet-induced HLP is related to the activation of PPARγ signaling pathway.
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