Cervical Transverse Process Tip Electroacupuncture Therapy Regulates Angiogenesis and Neurogenesis in Rats with Posterior Circulation Ischemia
[Objective]To observe the effects of cervical transverse process tip electroacupuncture therapy on cerebral blood flow and protein expression of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),matrix metalloproteinase-9(MMP-9),endostatin(ES)and Nestin,NeuN in rats with posterior circulation ischemia,and to investigate the effects of cervical transverse process tip electroacupuncture therapy on angiogenesis and nerve regeneration in rats with posterior circulation ischemia and the specific mechanism of the study.[Methods]Thirty-two male SD rats were replicated into a permanent posterior circulation ischemia model by ligating the right common carotid artery and the right subclavian artery,8 rats in the sham-operated group were only exposed without ligating the vessels,and 24 rats with successful modeling were divided into model group,western medicine group and cervical transverse process tip electroacupuncture group,8 rats in each group.The model group did not receive any acupuncture treatment,but the rats were grasped,bound and fixed at the same time to ensure that the time and conditions were the same as those in cervical transverse process tip electroacupuncture group;the rats in cervical transverse process tip electroacupuncture group were given electroacupuncture treatment to the Fengchi points on the sides and the cervical vertebral apices of the C2,C4 and C6 vertebrae;and western medicine group was treated by gavage with nimodipine solution.The frequency of treatment was 1 time/day,6 consecutive days for 1 course of treatment,and 1 day of rest at the end of 1 course of treatment,for a total of 2 courses of treatment.The mean cerebral blood flow in rats was measured by ultrasonic Doppler flowmetry;the number of new capillaries in the ischemic cerebral cortex of each group of rats was detected by fluorescein isothiocyanate(FITC)-Dextran tracing method;the morphology of cerebral cortex in the ischemic area was observed by hematoxylin-eosin(HE)staining;the positive expression levels of Nestin and NeuN in the right striatum of rat brain was detected by immunofluorescence staining;and the protein expression levels of VEGF,bFGF,MMP-9 and ES were analyzed by Western blot.[Results]After intervention,compared with sham-operated group,model group rats showed significant decrease in cerebral blood flow(P<0.01),increase in the number of new capillaries(P<0.05),increase in the positive expression of Nestin and NeuN in the right striatum(P<0.05,P<0.01),and increase in the protein expression of VEGF,MMP-9,bFGF and ES in the cerebral cortex of the ischemic area(P<0.01,P<0.05).Compared with model group,cerebral blood flow was significantly increased(P<0.01),the intensity of new capillaries in the cerebral cortex of the ischemic area was increased(P<0.05),the positive expression of Nestin and NeuN in the right striatum of the brain was increased(P<0.05,P<0.01),the expression of VEGF and bFGF protein was increased(P<0.01)and the expression of MMP-9 and ES protein was decreased(P<0.01)in the cerebral cortex of the ischemic area in the other two groups.Compared with western medicine group,the positive expression of Nestin and NeuN was increased in the right striatum of rats in cervical transverse process tip electro-acupuncture group(P<0.05),and the rest of the indexes were not statistically significant(P>0.05).[Conclusion]The cervical transverse process tip electroacupuncture therapy can improve cerebral blood flow and the degree of neurological deficit in rats with posterior circulation ischemia and promote angiogenesis and neurogenesis in rats,and its mechanism of action may be related to the regulation of protein expression of factors related to angiogenesis.
posterior circulation ischemiaacupuncturecervical transverse process tip electroacupuncture therapyangiogenesiscerebral ischemianeurogenesis
杨丽芳、文林月、欧小燕、张寒、柳刚
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安徽中医药大学 合肥 230031
广州中医药大学研究生院
安徽中医药大学第二附属医院
后循环缺血 针刺 颈椎横突尖电项针法 血管新生 脑缺血 神经发生
安徽省高等学校自然科学研究项目全国中医药创新骨干人才培养项目安徽省重大疑难疾病中西医协同攻关项目全国名老中医药专家传承工作室建设项目杨骏传承工作室项目National Traditional Chinese Medicine Innovation Backbone Talent Training ProjectCollaborative Research Project of Chinese and Western Medicine for Major Difficult Diseases in Anhui ProvinceYANG Jun National Famous Elderly Chinese Medicine Experts Inheritance Workshop Project
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