Impact of Chushi Weiling Decoction on Treatment in Mice with Atopic Dermatitis and Expression of ERK1/2 and Claudin 1 of Tissue
[Objective]To explore treatment effect of Chushi Weiling Decoction(CSWLD)on skin tissue lesion of atopic dermatitis(AD)mice,and its impact on expression of Claudin 1 and extracellular signal-regulated kinase1/2(ERK1/2)in tissue.[Methods]Mice were induced by dinitro fluoro benzene to establish AD model,then randomly divided into model group(0.9%sodium chloride solution),low,medium,high CSWLD groups(5,10,20 mL·kg-1),and set blank group(0.9%sodium chloride solution),all groups took intragastrical administration for 1 week.After administration,the improvement of skin lesion and inflammation scores were evaluated.Enzyme-linked immunosorbent assay(ELISA)assay was used to detect contents of interleukin-33(IL-33),IL-6,IL-4,C-C motif chemokine ligand-5(CCL-5)and CCL-2.Collecting tissue of skin lesion,immunohistochemical staining was used to detect the content of IL-33,suppression of tumorigenicity 2(ST2)and Claudin 1,quantitative polymerase chain reaction(qPCR)and Western blot were used to detect the mRNA and protein expression of ERK1/2 and Claudin 1.[Results]Compared with blank group,model group mice skin was red and swollen,dry and scabbed severely,inflammation score was increased,contents of serum IL-33,IL-6,IL-4,CCL-5 and CCL-2 were increased,contents of IL-33 and ST2 in tissue were increased,Claudin 1 mRNA expression were decreased,ERK1/2 mRNA expression was increased,Claudin 1 protein expression was decreased,phosphorylated-ERK1/2(p-ERK1/2)and ERK1/2 ratio was increased(P<0.01).After the administration with different doses of CSWLD,the redness and swelling of mice skin were degraded,scab separated and symptoms relived.Compared with model group,inflammation scores of mice in medium and high CSWLD groups were decreased,contents of serum IL-33,IL-6,IL-4,CCL-5 and CCL-2 were decreased;in tissue,contents of IL-33 and ST2 were decreased,Claudin 1 mRNA expression was increased,ERK1/2 mRNA expression were decreased,Claudin 1 protein expression were increased,p-ERK1/2 and ERK1/2 ratio were reduced(P<0.05,P<0.01);while there's no statistical difference of results in low CSWLD group(P>0.05).[Conclusion]CSWLD reduced inflammatory factors,inhibited p-ERK1/2/ERK1/2 protein expression and promoted Claudin 1 protein expression in tissue to relieve skin lesion symptoms in AD mice.
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