[目的]探讨野菊花有效部位(effective parts of wild chrysanthemum,CYM.E)对氧嗪酸钾复合次黄嘌呤致高尿酸血症模型小鼠和高嘌呤饮食致高尿酸血症模型大鼠的作用,并对CYM.E的安全性进行评价.[方法](1)药物安全性实验:将小鼠分为对照组,别嘌醇组(15 mg/kg),苯溴马隆组(7.5 mg/kg),CYM.E低、中、高剂量组(30、60、90 mg/kg),灌胃给药2周,评价CYM.E给药的安全性.(2)氧嗪酸钾复合次黄嘌呤致高尿酸血症小鼠实验:将小鼠分为对照组,模型组,别嘌醇组(15 mg/kg),苯溴马隆组(7.5 mg/kg),CYM.E低、中、高剂量组(30、60、90 mg/kg),灌胃给药1周,检测各组小鼠血清中尿酸(uric acid,UA)、肌酐(creatinine,CR)、血尿素氮(blood urea nitrogen,BUN)、丙氨酸转氨酶(alanine aminotransferase,ALT)和天冬氨酸转氨酶(aspartate aminotransferase,AST)水平.(3)高嘌呤饮食致高尿酸血症大鼠实验:将大鼠分为对照组,模型组,别嘌醇组(10 mg/kg),苯溴马隆组(5 mg/kg),CYM.E低、中、高剂量组(15、30、60 mg/kg),灌胃给药5周,检测各组大鼠血清中UA、CR、BUN、ALT和AST水平.[结果]药物安全性实验证实,CYM.E不影响正常小鼠血清中UA与肝肾功能指标水平.氧嗪酸钾复合次黄嘌呤致高尿酸血症小鼠实验提示,CYM.E中、高剂量能够显著降低高尿酸血症小鼠血清UA水平(P<0.05,P<0.01).高嘌呤饮食致高尿酸血症大鼠实验发现,给药3周后,高剂量CYM.E能够显著降低大鼠血清UA水平(P<0.01);给药5周后,中、高剂量CYM.E能够显著降低大鼠血清UA水平(P<0.05,P<0.01),高剂量能够显著降低大鼠血清CR、BUN水平(P<0.01,P<0.05),中剂量能够显著降低大鼠血清AST水平(P<0.05).[结论]CYM.E不影响正常小鼠的UA水平,未见相关肝肾损伤,达到一定剂量后,对两种高尿酸血症动物模型均能够较好地降低UA水平,且具有一定的肝肾功能保护作用.
Study on the Effect of Effective Parts of Wild Chrysanthemum on Two Kinds of Hyperuricemic Animals and Safety Evaluation
[Objective]To investigate the effects of effective parts of wild chrysanthemum(CYM.E)on potassium oxonate complex hypoxanthine-induced hyperuricemia in mice and high purine diet-induced hyperuricemia in rats and to evaluate the safety of CYM.E.[Methods](1)Drug safety experiments:Mice were divided into control group,allopurinol group(15 mg/kg),benzbromarone group(7.5 mg/kg),and CYM.E low,medium and high dose groups(30,60 and 90 mg/kg),and were administered by gavage for 2 weeks to evaluate the safety of CYM.E administration.(2)Experiments on hyperuricemia mice caused by hypoxanthine compounded with potassium oxonate:Mice were divided into control group,model group,allopurinol group(15 mg/kg),benzbromarone group(7.5 mg/kg),and CYM.E low,medium,and high dose groups(30,60 and 90 mg/kg),and administered by gavage for 1 week,and the serum levels of uric acid(UA),creatinine(CR),blood urea nitrogen(BUN),alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were detected.(3)Experiments on hyperuricemia rats caused by a high-purine diet:The rats were divided into control group,model group,allopurinol group(10 mg/kg),benzbromarone group(5 mg/kg),and CYM.E low,medium,and high dose groups(15,30 and 60 mg/kg),and were administered by gavage for 5 weeks,and the serum levels of UA,CR,BUN,ALT and AST were detected in each group.[Results]CYM.E did not affect serum UA levels and liver and kidney function indices in normal mice in drug safety experiments.The experiments of potassium oxonate combined with hypoxanthine in hyperuricemic mice indicated the medium and high doses of CYM.E could significantly reduce the serum UA level of hyperuricemic mice(P<0.05,P<0.01).The experiment of rats with hyperuricemia caused by high-purine diet showed,after 3 weeks of administration,the high dose of CYM.E could significantly reduce serum UA level(P<0.01);after 5 weeks of administration,the medium and high doses of CYM.E could significantly reduce the serum UA level(P<0.05,P<0.01),and the high dose of CYM.E could significantly reduce the serum CR and BUN levels(P<0.01,P<0.05),and the medium dose could significantly reduce the serum AST level(P<0.05).[Conclusion]CYM.E did not affect the UA level of normal mice,and no related liver and kidney injury was seen;and after reaching a certain dose,it had a better uric acid-lowering effect on both models of hyperuricemia,and had a certain protective effect on liver and kidney functions.
effective parts of wild chrysanthemumhyperuricemiaanimal modelserumuric acidliver functionkidney functionsecurity
万方数据
维普
