Effect and Mechanism of Cnidium monnieri on Osteoporosis in Kidney Yang Deficiency-induced Osteoporotic Rats
[Objective]To investigate the osteoprotective effect and molecular mechanism of Cnidium monnieri on kidney Yang deficiency-induced osteoporosis in rats.[Methods]SD rats were divided into normal,model,Cnidium monnieri extract and osthole groups.The rats except for normal group were given hydrocortisone subcutaneously every day.The rats were given Cnidium Monnieri extract,osthole or vehicle intragastric daily for 3 weeks.After anesthesia,rat femurs were collected.Tartrate phosphatase staining,transforming growth faction-β(TGF-β)immunohistochemical staining and bone mineral density detection were performed on the left femur,while Real-time quantitative polymerase chain reaction and western blot detection of target gene and protein expression were performed on the right femur.[Results]Hydrocortisone has been shown to significantly enhance the differentiation and maturation of rat osteoclasts,while inhibiting the formation of osteoblasts.This results in a reduction in the formation of bone trabeculae and ultimately induces osteoporosis in rats with kidney-yang deficiency.The results of bioinformatics analysis suggested that TGF-β signaling pathway was the potential candidate target pathway for Cnidium monnieri.Cnidium monnieri extract and osthole significantly reduced the differentiation and maturation of osteoclasts,promoted the differentiation and maturation of osteoblasts,and ultimately inhibited the osteoporosis in rats induced by hydrocortisone.The extract of Cnidium monnieri and osthole significantly increased the downregulation of TGF-β gene induced by hydrocortisone in femur.[Conclusion]Cnidium monnieri and osthole can inhibit osteoporosis in kidneyYang deficiency rats through TGF-β signaling pathway.
Cnidium monnieriostholehydrocortisoneosteoporosiskidney Yang deficiencyTGF-β signaling pathwaymolecular mechanismtherapeutic effect
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