摘要
目的:研究三七皂苷血清外泌体对脑出血(ICH)大鼠脑组织病理形态学改变及炎症因子表达的影响.探讨对ICH大鼠脑组织炎症损伤的保护作用.方法:采用随机数字表法将30只造模成功大鼠分为三七皂苷血清外泌体组、三七皂苷组、模型组,分别予三七皂苷血清外泌体(20μg/day)、三七皂苷(100mg/kg)、等体积生理盐水进行尾静脉连续注射10天,同期设立正常对照组.实验结束后,比较4组大鼠脑组织病理损伤程度,采用RT-PCR检测脑出血损伤周围组织中炎症指标核转录因子κB(NF-κB)、炎症小体3(NLRP-3)、白介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)的mRNA表达水平,利用ELISA法检测脑组织匀浆中NF-κB、IL-6及IL1β含量.结果:与模型组比较,三七皂苷血清外泌体组、三七皂苷组均有不同程度的脑血肿吸收、炎性浸润减轻,且三七皂苷血清外泌体组的效果优于三七皂苷组.三七皂苷血清外泌体组炎症指标NF-κB、NLRP-3、IL-6、TNF-α及IL1β表达水平较模型组明显下调,且低于三七皂苷组,差异有统计学意义(P＜0.05).结论:三七皂苷血清外泌体作为生物活性物质可有效改善脑出血大鼠脑组织炎症水平,缓解病理损害,对脑出血损伤具有一定保护作用.
Abstract
Objective:To study the effects of Panax ginseng saponin serum exosomes on pathologi-cal morphological changes in brain tissue and expression of inflammatory factors in rats with Intra-cerebral Hemorrhage(ICH).Explore the protective effect on brain tissue inflammatory injury in ICH rats.Methods:Thirty successfully modelled rats were divided into Panax ginseng saponin se-rum exosome group,Panax ginseng saponin group,and model group by random number table meth-od,and were given Panax ginseng saponin serum exosome(20μg/day),Panax ginseng saponin(100mg/kg),and equal volume of physiological saline for tail vein injection for 10 days consecu-tively,meanwhile a normal rat group was set up for comparison.After the intervention,compare the degree of histopathological damage in the brain tissue of the four groups of rats.RT-PCR was used to detect the mRNA expression levels of inflammatory indexes NF-κB,NLRP3,IL-6 and TNF-α in the tissues surrounding the intracerebral hemorrhage injury.The levels of NF-κB,IL-6 and IL1β in homogenates of brain tissues were detected by using ELISA.Results:Compared with the model group,the Panax ginseng saponin serum exosome group and Panax ginseng saponin group had dif-ferent degrees of intracerebral hemorrhage resorption and reduction of inflammatory infiltration,and the effect of Panax ginseng saponin serum exosome group was better than that of Panax ginseng sa-ponin group.The expression levels of inflammatory indexes NF-κB,NLRP-3,IL-6,TNF-α and IL1β in Panax ginseng saponin serum exosome group were significantly down-regulated compared with that in model group and lower than that in Panax ginseng saponin group,and the difference was statistically significant(P<0.05).Conclusion:Panax ginseng saponin serum exosome as a bioactive substance can effectively improve the level of brain tissue inflammation after intracerebral hemor-rhage in intracerebral hemorrhage rats,alleviate pathological damage,and have a certain protective effect on intracerebral hemorrhage injury.
国家科技期刊平台
NSTL
维普
万方数据