Study on the Mechanism and Experimental Validation of Gegen Tang in Treating Migraine Based on Network Pharmacology
Objective:Exploring the mechanism of action of Gegen Tang in treating migraine based on network pharmacology and verifying it through cell experiments.Methods:Retrieve the chemical substances and drug targets of Gegen Tang through the traditional chinese medicine systems phar-macology database and analysis platform,and search for disease targets of migraine through the Genecard database.Intersecting the two targets to obtain potential targets for treating migraine with Gegen Tang.Construct effective compound target disease network and PPI network.Conduct GO func-tion and KEGG pathway enrichment analysis using Bioconductor bioinformatics software package.Cell experiments were conducted using BV-2 microglial cells,divided into blank control group(control),model group(LPS group),and Gegen Tang Gao(800 μ g·mL-1),low-dose group(400 μ g·mL-1).Results:A total of 122 potential effective ingredients and 144 potential targets were screened for the treatment of migraine with Gegen Tang.Quercetin,luteolin,and kaempferol may be key components,while STAT3,JUN,and IL6 may be key targets.Oxidative stress response,DNA signal transcription transduction,receptor binding,cytokine activity,etc.are possible biological pathways for the treatment of migraine with Gegen Tang.AGE-RAGE,lipid and atherosclerosis,TNF,IL-17 and other signal pathways are the possible signal pathways of Gegen Decoction in treating migraine.Cell experiments showed that the survival rate of cells in the 1.6,2.0,and 4.0 mg·mL-1 groups of Gegen Tang decoction significantly decreased(P<0.01),while there was no statistically significant difference in the survival rate of cells in the 0.2,0.4,and 0.8 mg·mL-1 groups of Ge-gen Tang decoction(P>0.05).Western blot results showed that compared with the blank control group,the expression of IL-6,STAT3,and p-STAT3 proteins in the model group was significantly increased(P<0.05).Compared with the model group,the high-dose and low-dose groups of Gegen Tang significantly reduced the expression of IL-6,STAT3,and p-STAT3 proteins(P<0.05).Conclu-sion:Quercetin,luteolin,kaempferol,etc.in Gegen Decoction may play a role in the treatment of mi-graine by acting on STAT3,IL-6 and other targets to regulate lipid and atherosclerosis pathways.
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