Anti-cancer Fuzheng Formula Inhibits Hepatocellular Carcinoma Progression by Regulating Autophagy and JAK2/STAT3 Signaling Pathway
Objective:This study aimed to investigate the effect of anti-cancer FuZheng formula to regulate cell autophagy on the progression of hepatocellular carcinoma(HCC).Methods:Human hepatoma cells SMMC-7721 were treated with anti-cancer FuZheng formula(0,1,2 mg/mL)and cisplatin(4 mg/L),respectively,and divided into control group,anti-cancer Fuzheng formula low and high dose group,and cisplatin group.Meanwhile,SMMC-7721 cells were divided into the anti-cancer Fuzheng formula group(2 mg/mL),anti-cancer Fuzheng formula(2 mg/mL)+ 3-methylad-enine(3-Methyladenine,3-MA,2 mmol/L)group,and the control group(equal amount of saline).And incubated in the cell culture incubator for 48 h after administration of drugs according to the group.MTT,flow MDC assay were performed to detect inhibition rate and autophagy rate of SMMC-7721 cells.Western blot was performed to detect the expression levels of autophagy-related molecu-lar proteins and JAK2/STAT3 signaling pathway-related proteins in SMMC-7721 cells.Results:Com-pared with the control group,the inhibition rate and autophagy rate of SMMC-7721 cells in the low and high dose groups of anti-cancer Fu ZhengFang formula and cisplatin group were signifi-cantly higher,and the expression of Beclin-1 and LC3 proteins were significantly increased and P62 protein expression was significantly decreased(P＜0.05);SMMC-7721 cells in the high dose group of anti-cancer FuZheng formula had significantly higher P-JAK-2/JAK-2 and P-STAT3/STAT3 protein expression was significantly decreased in SMMC-7721 cells at high dose(P＜0.01).While the inhibition rate and autophagy rate of SMMC-7721 cells were significantly lower in the Anti-Cancer Fu Zheng formula + 3-MA group compared with the anti-Cancer Fu Zheng formula(P＜0.01).Conclusion:Anti-cancer Fuzheng Formula up-regulates Beclin-1 and LC3 proteins,down-regulates P62 protein to enhance cellular autophagy,and inhibits JAK2/STAT3 signaling path-way,together interfering with HCC progression.
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