Objective:To explore the effect and mechanism of Tianzhi granules on Aβ25-35-induced pheochromocytoma PC12 cells apoptosis.Method:PC12 cells were induced by Aβ25-35 in the pres-ence or absence of different dose of Tianzhi granules without or with LY294002.The cell viability was determined by MTT,apoptosis and oxidative stress were measured by flow cytometry,caspase 3 activity and MDA content were detected by microplate reader.The levels of Bcl2,Bax,p-AKT and AKT protein were determined by western blotting.Results:Tianzhi granules dose-dependently im-proved Aβ25-35-induced PC12 cells apoptosis,as indicated by increased cell viability,decreased cas-pase 3 activity,as well as upregulating Bcl2/Bax.Furthermore,Tianzhi granules dose-dependently in-hibited Aβ25-35-induced oxidative stress and MDA content but activated PI3K/AKT signal pathway,and the inhibitor of PI3K/AKT,LY294002,reversed the beneficial effects of Tianzhi granules in Aβ25-35-primed PC12 cells.Conclusion:Tianzhi granules could effectively protect from Aβ25-35-primed PC12 cells,which might be mediated by activating PI3K/AKT pathway.
关键词
天智颗粒/阿尔茨海默病/PC12细胞/凋亡/PI3K-AKT信号
Key words
Tianzhi Granules/alzheimer disease/PC12 cells/apoptosis/PI3K/AKT signal
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