Study of Stachyrine Hydrochlorid Combined with Cross-linked Sodium Hyaluronate Gel in the Fibrosis of Intrauterine Adhesions Rabbit Model
Objective:Evaluate the therapeutic effect of stachyrine hydrochlorid combined with cross-linked so-dium hyaluronate gel on intrauterine adhesions(IUA)in rabbits and the relevant mechanism of action.Meth-ods:Rabbits were randomLy divided into three groups:sham-operated group、model group、experimental group.IUA rabbit model was induced by mechanical damages.Experimental group were perfusion stachyrine hy-drochlorid(Sta)combined with cross-linked sodium hyaluronate gel to the uterine cavity during the surgery,and injected Sta after the surgery.The histological changes of the uterus were observed after 2-week medication,compared with the number of the endometrial glands and blood vessel mass,the fibrosis area in each group.The expressions of Smad4、TGF-β1、TIMP-1、MMP-9 and VEGF1were detected using West blot.Resuits:Compared with sham-operated group,the number of the endometrial glands and blood vessel mass were reduced.the fibrosis area were increased(P＜0.05),the expression of Smad4、TGF-β1 and TIMP-1 were elevaled the expression of MMP-9 decreased in model group(P＜0.05).Compared with model group,he num-ber of the endometrial glands and blood vessel mass were increased.the fibrosis area were reduced(P＜0.05),the expression of Smad4、TGF-β1、TIMP-1and VEGF1 were decreased,the expression of MMP-9 elevaled in experimental group(P＜0.05).Conclusion:Sta combined with cross-linked sodium hyaluronate gel can improve endometrial fibrosis in IUA rabbits,and its mechanism may be related to the anti-fibrotic ac-tions and neovascularization.
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