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墨兰和蝴蝶兰镰刀菌病害的鉴定

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对广东栽培墨兰(Cymbidium sinense (Andr.) Willd.)和蝴蝶兰(Phalaenopsis amabilis Blume)的镰刀菌病害进行了鉴定.经症状观察、病原菌分离培养与回接、病原菌形态观察和内部转录间隔区(Internal transcribed space,ITS)、延伸因子-1α(Elongation factor-1α,EF-1α)序列分析,鉴定出墨兰茎腐病、蝴蝶兰茎基腐病和蝴蝶兰花梗腐烂病等3种镰刀菌病害,其病原菌分别为尖孢镰刀菌(Fusarium oxysporum)、茄镰刀菌(F.solani)和藤仓镰刀菌(F.Fujikuroi).其中,蝴蝶兰花梗腐烂病(F.fujikuroi)为兰花上首次报道.
Identification of Fusarium diseases on Cymbidium sinense and Phalaenopsis amabilis
Fusarium diseases on Cymbidium sinense and Phalaenopsis amabilis in Guangdong were identified.After the pathogenicity of single-conidium isolates was verified,pathogens were identified via morphological observation and sequence analyses of ITS and EF-1α.The results showed that pseudobulb rot of C.sinense was caused by Fusarium oxysporum,and that leaf butt rot and flower stalk rot of P.amabilis were caused by F.solani and by F.fujikuroi,respectively.This is the first report of F.fujikuroi as a pathogen on moth orchid.

fungal diseasesCymbidium sinensePhalaenopsis amabilisFusarium

施祖荣、张云霞、黄江华、向梅梅

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仲恺农业工程学院农学院,广东广州510225

真菌病害 墨兰(Cymbidium sinense (Andr.) Willd.) 蝴蝶兰(Phalaenopsis amabilis Blume) 镰刀菌(Fusarium)

广东省花卉产业技术体系建设专项项目仲恺农业工程学院研究生创新基金

2060302H1409011

2011

仲恺农业工程学院学报
仲恺农业工程学院

仲恺农业工程学院学报

影响因子:0.292
ISSN:1674-5663
年,卷(期):2011.24(2)
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