Community composition and diversity of endophytic fungi in leaves,fruits and branches of different Eucalyptus spp.
Endophytic fungal communities have different compositions and distributions in different tis-sues of different plants.The community composition and diversity of endophytic fungi in leaves,fruits and branches of four Eucalyptus species were compared by ITS high-throughput sequencing technique.The results of endophytic fungal communities structure analysis of four Eucalyptus species showed that As-comycota and Basidiomycota were the dominant phyla in all samples,but their relative abundance varied in different samples.The results of biodiversity analysis illustrated that the endophytic fungal communities of Eucalyptus torelliana and E.citriodora were similar in the same tissue and in different tissues,while E.exserta and G9 had similar endophytic fungal communities in the same tissue but with differences in different tissues.The diversity indices of the four Eucalyptus species in branches were not significantly different,while the Chaol,Observed species and PD whole tree indices of endophytic fungal communities were significantly higher in the fruits of E.exserta and G9 than in E.torelliana and E.citriodora.LEfSe analysis showed that the differential species in E.citriodora(branches and fruits)and E.exserta(leaves and fruits)were distributed in the Ascomycota,while those in E.torelliana(fruits)and G9(leaves and branches)were distributed in both Ascomycota and Basidiomycota.Eucalyptus spp.contains abundant endophytic fungal flora,which varies greatly in different species and tissues.There are more abundant endophytic fungi community in the leaves of G9 and fruits of E.exserta.This study provides an important basis for the development and utilization of endophytic fungal resources in Eucalyptus spp..
endophytic fungi of Eucalyptusendophytic fungal communitiescommunity structurebiodiversityhigh-throughput sequencing