仲恺农业工程学院学报2024,Vol.37Issue(1) :16-25,31.DOI:10.3969/j.issn.1674-5663.2024.01.003

壳寡糖接枝玉米醇溶蛋白/芸苔素内酯纳米粒子的制备及抗盐胁迫性能

Preparation of chitosan oligosaccharide grafted zein/brassinolide nanoparticles with salt stress resistance

林依彤 韩韦 黄志锋 周红军 周新华
仲恺农业工程学院学报2024,Vol.37Issue(1) :16-25,31.DOI:10.3969/j.issn.1674-5663.2024.01.003

壳寡糖接枝玉米醇溶蛋白/芸苔素内酯纳米粒子的制备及抗盐胁迫性能

Preparation of chitosan oligosaccharide grafted zein/brassinolide nanoparticles with salt stress resistance

林依彤 1韩韦 1黄志锋 2周红军 1周新华1
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作者信息

  • 1. 仲恺农业工程学院化学化工学院,广东广州 510225;仲恺农业工程学院广东省普通高校农业绿色精细化学品重点实验室,广东广州 510225
  • 2. 仲恺农业工程学院化学化工学院,广东广州 510225
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摘要

为解决芸苔素内酯(Brassinolide,BR)溶解性差、易被植物代谢且存在浓度依赖性、生物利用度低等问题,构建了由亲水性多糖壳寡糖(Chitosan Oligosaccharide,COS)和乙二醇二缩水甘油醚(Ethylene Glycol Diglycidyl Ether,EGDE)修饰玉米醇溶蛋白(Z-COS)负载芸苔素内酯(BR@Z-COS)的纳米体系.研究了 Z-COS的结构、形貌特点以及BR@Z-COS对黄瓜种子发芽和幼苗在盐胁迫下的影响.结果表明,Z-COS在水中的溶解度为13%,BR@Z-COS的平均粒径为98 nm,PDI值少于0.2;Z-COS对BR有一定的缓释效果,对BR的包封率和载药量分别为17.81%和6.85μg/mg.当BR@Z-COS的浓度为0.1 mg/L时,可以增加植物体内的脯氨酸含量,有效缓解盐胁迫下的植株生长发育遭受的损害.

Abstract

In order to solve the problems of poor solubility,easy metabolism by plants,concentration de-pendence and low bioavailability of brassinolide(BR),a nanosystem of zein(Z-COS)modified with chi-tosan oligosaccharide(COS)and ethylene glycol diglycidyl ether(EGDE)to load BR(BR@Z-COS)was constructed.The structure and morphology of Z-COS and the effects of BR@Z-COS on cucumber seed germination and seedling under salt stress were studied.The results showed that the solubility of Z-COS in water reached 13%,the average particle size of BR@Z-COS was 98 nm,and the PDI value was less than 0.2.Z-COS had a certain sustained release effect on BR,and the encapsulation efficiency and drug loading of BR reached 17.81%and 6.85 μg/mg,respectively.When the concentration of BR@Z-COS was 0.1 mg/L,the proline content in plants could be increased,thus effectively alleviating the damage to plant growth under salt stress environment.

关键词

玉米醇溶蛋白/壳寡糖/芸苔素内酯/盐胁迫

Key words

zein/chitosan oligosaccharide/brassinolide/salt stress

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基金项目

广东省国际科技合作项目(2021A0505030073)

广东省教育厅提升项目(2022ZDJS020)

广东省重点领域研发计划(2023B02028002)

出版年

2024
仲恺农业工程学院学报
仲恺农业工程学院

仲恺农业工程学院学报

影响因子:0.292
ISSN:1674-5663
参考文献量36
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