Preparation of chitosan oligosaccharide grafted zein/brassinolide nanoparticles with salt stress resistance
In order to solve the problems of poor solubility,easy metabolism by plants,concentration de-pendence and low bioavailability of brassinolide(BR),a nanosystem of zein(Z-COS)modified with chi-tosan oligosaccharide(COS)and ethylene glycol diglycidyl ether(EGDE)to load BR(BR@Z-COS)was constructed.The structure and morphology of Z-COS and the effects of BR@Z-COS on cucumber seed germination and seedling under salt stress were studied.The results showed that the solubility of Z-COS in water reached 13％,the average particle size of BR@Z-COS was 98 nm,and the PDI value was less than 0.2.Z-COS had a certain sustained release effect on BR,and the encapsulation efficiency and drug loading of BR reached 17.81％and 6.85 μg/mg,respectively.When the concentration of BR@Z-COS was 0.1 mg/L,the proline content in plants could be increased,thus effectively alleviating the damage to plant growth under salt stress environment.
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