首页|高效输导咯菌腈纳米杀菌剂的制备及其性能研究

高效输导咯菌腈纳米杀菌剂的制备及其性能研究

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为提高杀菌剂对香蕉枯萎病病灶部位的靶向积累,以苯丙氨酸(Phenylalanine,PHE)和聚琥珀酰亚胺(Polysuccinimide,PSI)为原料,构建靶向高分子纳米载体(PSI-PHE)负载咯菌腈(Fludioxonil,FLU),制备出苯丙氨酸介导的咯菌腈纳米杀菌剂(FLU@PSI-PHE).采用红外光谱、激光粒度仪、热重分析和X射线衍射手段等表征了原药与高分子载体之间的互作关系及载药率.研究结果表明,水合粒径约531 nm的FLU@PSI-PHE纳米颗粒载药率高达45.96%,其在蓖麻韧皮部的输导剂量为1.27 μg/mL,显示出高效韧皮部输导效果.对比低剂量FLU原药,活性成分含量相同的FLU@PSI-PHE纳米颗粒对香蕉枯萎病的抑菌活性基本相当.氨基酸介导的咯菌腈纳米杀菌剂在植物维管组织中高效输导,为香蕉枯萎病的靶向治疗提供了新的技术手段.
Preparation and properties of efficiently translocated fludioxonil nanofungicide
In order to improve the targeted accumulation of fungicides on the lesion locations against Fusarium wilt in bananas,polymer nanocarriers(PSI-PHE)were constructed with polysuccinimide(PSI)and phenylalanine(PHE).Then fludioxonil(FLU)was loaded into the nanocarriers to produce PHE-guided nanoparticles(FLU@PSI-PHE).The infrared spectroscopy,hydrodynamic size,zeta potential,thermogravimetric analysis and X-ray diffraction analysis were explored to characterize the in-teraction and loading capacity between nanocarriers and FLU.The results showed that the loading capaci-ty of FLU@PSI-PHE nanoparticles with 531 nm by hydrodynamic size was up to 45.96%,and the trans-location dose of the castor phloem was 1.27 μg/mL,showing efficient phloem delivery effect.Compared with low doses of FLU,FLU@PSI-PHE nanoparticles with the same active ingredient content showed ba-sically equal antifungal activity against Fusarium wilt in bananas.Amino acid-mediated FLU@PSI-PHE could be efficiently translocated in plant vascular tissues,which provides new technology for targeted treatment of banana Fusarium wilt.

amino acidtranslocationbanana Fusarium wiltnanocarriers

黄炜柔、何亮亨、朱丽、邓文杰、贾金亮

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华南农业大学材料与能源学院,广东广州 510642

氨基酸 输导 香蕉枯萎病 纳米载体

广东省重点领域研发计划广东省自然科学基金广东省现代农业产业技术体系创新团队专项

2023B02020800012023A15150106092019KJ140

2024

仲恺农业工程学院学报
仲恺农业工程学院

仲恺农业工程学院学报

影响因子:0.292
ISSN:1674-5663
年,卷(期):2024.37(1)
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