仲恺农业工程学院学报2024,Vol.37Issue(1) :26-31.DOI:10.3969/j.issn.1674-5663.2024.01.004

高效输导咯菌腈纳米杀菌剂的制备及其性能研究

Preparation and properties of efficiently translocated fludioxonil nanofungicide

黄炜柔 何亮亨 朱丽 邓文杰 贾金亮
仲恺农业工程学院学报2024,Vol.37Issue(1) :26-31.DOI:10.3969/j.issn.1674-5663.2024.01.004

高效输导咯菌腈纳米杀菌剂的制备及其性能研究

Preparation and properties of efficiently translocated fludioxonil nanofungicide

黄炜柔 1何亮亨 1朱丽 1邓文杰 1贾金亮1
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作者信息

  • 1. 华南农业大学材料与能源学院,广东广州 510642
  • 折叠

摘要

为提高杀菌剂对香蕉枯萎病病灶部位的靶向积累,以苯丙氨酸(Phenylalanine,PHE)和聚琥珀酰亚胺(Polysuccinimide,PSI)为原料,构建靶向高分子纳米载体(PSI-PHE)负载咯菌腈(Fludioxonil,FLU),制备出苯丙氨酸介导的咯菌腈纳米杀菌剂(FLU@PSI-PHE).采用红外光谱、激光粒度仪、热重分析和X射线衍射手段等表征了原药与高分子载体之间的互作关系及载药率.研究结果表明,水合粒径约531 nm的FLU@PSI-PHE纳米颗粒载药率高达45.96%,其在蓖麻韧皮部的输导剂量为1.27 μg/mL,显示出高效韧皮部输导效果.对比低剂量FLU原药,活性成分含量相同的FLU@PSI-PHE纳米颗粒对香蕉枯萎病的抑菌活性基本相当.氨基酸介导的咯菌腈纳米杀菌剂在植物维管组织中高效输导,为香蕉枯萎病的靶向治疗提供了新的技术手段.

Abstract

In order to improve the targeted accumulation of fungicides on the lesion locations against Fusarium wilt in bananas,polymer nanocarriers(PSI-PHE)were constructed with polysuccinimide(PSI)and phenylalanine(PHE).Then fludioxonil(FLU)was loaded into the nanocarriers to produce PHE-guided nanoparticles(FLU@PSI-PHE).The infrared spectroscopy,hydrodynamic size,zeta potential,thermogravimetric analysis and X-ray diffraction analysis were explored to characterize the in-teraction and loading capacity between nanocarriers and FLU.The results showed that the loading capaci-ty of FLU@PSI-PHE nanoparticles with 531 nm by hydrodynamic size was up to 45.96%,and the trans-location dose of the castor phloem was 1.27 μg/mL,showing efficient phloem delivery effect.Compared with low doses of FLU,FLU@PSI-PHE nanoparticles with the same active ingredient content showed ba-sically equal antifungal activity against Fusarium wilt in bananas.Amino acid-mediated FLU@PSI-PHE could be efficiently translocated in plant vascular tissues,which provides new technology for targeted treatment of banana Fusarium wilt.

关键词

氨基酸/输导/香蕉枯萎病/纳米载体

Key words

amino acid/translocation/banana Fusarium wilt/nanocarriers

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基金项目

广东省重点领域研发计划(2023B0202080001)

广东省自然科学基金(2023A1515010609)

广东省现代农业产业技术体系创新团队专项(2019KJ140)

出版年

2024
仲恺农业工程学院学报
仲恺农业工程学院

仲恺农业工程学院学报

影响因子:0.292
ISSN:1674-5663
参考文献量16
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