Isolation,identification and degradation characteristics of a fusaric acid degrading bacterium
In order to provide strain resources for detoxification of fusaric acid(FA)-contaminated soil and feed,soil samples were collected from banana rhizosphere soil in the field where fusarium wilt oc-curred,An FA degrading bacterium FAD1 was isolated by enrichment culture.Through the observation of colony morphological characteristics and 16S rDNA gene sequence alignment,the strain FAD1 was identified as Mycobacterium sp.,FAD1 was inoculated into the medium with FA as the sole carbon(C)or nitrogen(N)source to observe its growth characteristics.The degradation effect of FAD1 on FA was evaluated by high performance liquid chromatography(HPLC)and plant in vivo test.The results showed that FAD1 could grow in the medium with FA as the sole source of C or N,and the degradation effect of FA was the best in the medium with FA as the sole source of N or additional sources of C and N.The HPLC results showed that the degradation rate of FA was up to 89％when FAD1 was cultured in FA con-taining medium until the decline phase.Plant in vivo test showed that tomato(Solanum lycopersicum)seedlings lost turgid pressure and died of leaf water loss after soaking in MMF medium containing FA without FAD1 fermentation for 40 h,while seedlings treated after FAD1 fermentation survived.In conclu-sion,FAD1 can grow in the culture medium with FA as the sole source of C and N.FAD1 can degrade FA to a certain degree,which has a application value in preventing the harm of FA to plants,animals and microorganisms.
万方数据
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