The aim of this study was to optimize the extraction process of total flavonoids from Annona squamosa leaves and study their antioxidant activity in vitro.Using ethanol as extraction solvent,the re-sponse surface methodology was used to optimize the extraction process of total flavonoids from lychee leaves based on the results of single factor experiments.DPPH,-OH and ABTS were used as indicators to comprehensively evaluate the in vitro antioxidant activity of total flavonoids from lychee leaves.The re-sults showed that the optimal extraction parameters were:Ethanol volume fraction 70％,liquid to materi-al ratio 50∶1(mL∶g),ultrasonic time 85 minutes,ultrasonic temperature 67 ℃.The extraction rate of total flavonoids was 4.37％under this process.The antioxidant activity of total flavonoids was evaluated by three indexes(the scavenging ability of DPPH,-OH and ABTS).The results showed that total flavonoids from Annona squamosa leaves had good scavenging effects on DPPH,-OH and ABTS,with the IC50 values were 94.78 μg/mL,756.34 μg/mL and 419.91 μg/mL,respectively.And the scavenging effect on-OH was stronger than that of VC.The process determined in this study was simple and feasible.The total flavonoids extracted from Annona squamosa leaves had high extraction rate and strong antioxi-dant capacity.
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