滚环扩增(rolling circle amplification,RCA)是一种以环状DNA单链为模板,由DNA聚合酶介导的高效等温酶促反应,具有简便、快速、高效等优点,能在短时间内快速产生大量具有重复碱基序列的超长单链DNA.由于DNA的可编码性,可以根据需要去设计模板DNA序列,从而产生大量功能性的产物,在生物检测、药物递送等领域发挥出独特优势.本文概述了RCA技术的基本原理与分类,并着重介绍了近几年RCA技术在肿瘤检测及药物递送方面的应用,最后讨论了该技术的应用前景和所面临的挑战.
Abstract
Rolling circle amplification(RCA)is an efficient isothermal enzymatic reaction using a circular DNA single strand as a template and catalyzed by DNA polymerases.It is simple,fast,and efficient,and can rapidly produce ultra-long single-stranded DNA with a large number of tandem repeats in a short time.Due to the codability of DNA,template DNA sequences can be designed according to needs to result in a wide range of functional products,displaying a unique advantage in the fields of biological detection and drug delivery.This review has summarized the basic principle and classification of RCA technology,focused on the applications of RCA technology in tumor detection and drug delivery in recent years,and finally discussed the application prospects and potential challenges of this technology.
关键词
滚环扩增/肿瘤标志物/肿瘤早期检测/药物递送系统
Key words
Rolling circle amplification/Tumor markers/Early detection of cancer/Drug delivery systems
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